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AB123166

Recombinant Human PGK2 protein (His tag N-Terminus)

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(2 제품이 사용된 논문 )

Recombinant Human PGK2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 417 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

PGKB, PGK2, Phosphoglycerate kinase 2

1 이미지
SDS-PAGE - Recombinant Human PGK2 protein (His tag N-Terminus) (AB123166)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PGK2 protein (His tag N-Terminus) (AB123166)

15% SDS-PAGE showing ab123166 (3µg).

주요 정보

Purity

>85% SDS-PAGE

ab123166 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Proteins & Peptides

AB132692

Recombinant Human GPD2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human GPD2 protein (GST tag N-Terminus) (AB132692)

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  • 0
Proteins & Peptides

AB116799

Recombinant Human GPI-PLD protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human GPI-PLD protein (GST tag N-Terminus) (AB116799)

  • 0
  • 0
Proteins & Peptides

AB318253

Recombinant Human HSP90 alpha Protein (His-tag)

Mass Spectrometry - Recombinant Human HSP90 alpha Protein (His-tag) (AB318253)

  • 0
  • 0
Primary Antibodies

AB188585

Anti-GPD2 antibody [EPR14259]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (AB188585)

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  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM","proteinLength":"Full Length","predictedMolecularWeight":"46.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":417,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P07205","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglycerate kinase 2 (PGK2) also known as testis-specific phosphoglycerate kinase is an enzyme with a molecular mass of approximately 45 kDa. It functions in glycolysis by catalyzing the reversible conversion of 13-bisphosphoglycerate to 3-phosphoglycerate producing ATP in the process. PGK2 expression is restricted mostly to the testes where it plays an important role in sperm development and motility. Unlike its isozyme counterpart PGK1 which is expressed ubiquitously PGK2 is testis-specific.
Biological function summary

The enzyme participates in the important processes of energy production and spermatozoa development. While PGK2 does not form part of any multi-protein complex it is an integral component of the glycolytic pathway contributing to ATP production necessary for sperm motility. Its expression is regulated during spermatogenesis highlighting its role in male fertility.

Pathways

PGK2 operates within the glycolytic pathway a major metabolic route for energy production. Along with glyceraldehyde-3-phosphate dehydrogenase PGK2 catalyzes important reactions that facilitate the conversion of glucose to pyruvate yielding high-energy ATP molecules. By functioning in tandem with other glycolytic enzymes PGK2 ensures the efficient production of energy needed for cellular functions especially during spermatogenesis.

Defects or alterations in PGK2 have associations with male infertility notably due to impaired sperm function. Disorders like spermatogenic failure and related conditions arise when PGK2 activity diminishes. Its enzyme partner glyceraldehyde-3-phosphate dehydrogenase-S is also linked to sperm motility issues. Understanding PGK2’s role aids in identifying therapeutic targets to address such reproductive challenges.
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일반 정보

기능

Catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate and plays a role in glycolysis and gluconeogenesis (By similarity). Essential for sperm motility and male fertility (PubMed : 26677959). Not required for the completion of spermatogenesis (By similarity).

서열 유사성

Belongs to the phosphoglycerate kinase family.

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제품 프로토콜

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타겟 정보

Catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate and plays a role in glycolysis and gluconeogenesis (By similarity). Essential for sperm motility and male fertility (PubMed : 26677959). Not required for the completion of spermatogenesis (By similarity).
See full target information PGK2
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 19:e0310530 PubMed39509399

2024

Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyao Ruan,Chen Wang,Jiawen Gu,Zhihui Zhu

Frontiers in pharmacology 11:379 PubMed32292350

2020

Esculetin Inhibits Cancer Cell Glycolysis by Binding Tumor PGK2, GPD2, and GPI.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Tao Wu,Bo Liu,Zhong-Zhu Ai,Zong-Chao Hong,Peng-Tao You,He-Zhen Wu,Yan-Fang Yang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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