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AB161607

Recombinant Human PHF15 protein (GST tag N-Terminus)

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Recombinant Human PHF15 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 246 aa range, expressed in Wheat germ, suitable for ELISA, WB.

대체 명칭 보기

KIAA0239, PHF15, JADE2, E3 ubiquitin-protein ligase Jade-2, Jade family PHD finger protein 2, PHD finger protein 15

1 이미지
SDS-PAGE - Recombinant Human PHF15 protein (GST tag N-Terminus) (AB161607)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PHF15 protein (GST tag N-Terminus) (AB161607)

ab161607 on a 12.5% SDS-PAGE stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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보완 제품

Primary Antibodies

AB129495

Anti-PHF16 antibody

Immunoprecipitation - Anti-PHF16 antibody (AB129495)

  • 0
  • 0
Primary Antibodies

AB66968

Anti-PHF17 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody (AB66968)

  • 0
  • 0
Primary Antibodies

AB124929

Anti-Mib1/Mindbomb antibody [EPR2762(2)]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

  • 4
  • 4
Primary Antibodies

AB21286

Anti-Collagen I antibody

Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody (AB21286)

  • 4
  • 16
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MAMSEWPLNNGHREDPAPGLLSEELLQDEETLLSFMRDPSLRPGDPARKARGRTRLPAKKKPPPPPPQDGPGSRTTPDKAPKKTWGQDAGSGKGGQGPPTRKPPRRTSSHLPSSPAAGDCPILATPESPPPLAPETPDEAASVAADSDVQVPGPAASPKPLGRLRPPRESKVTRRLPGARPDAGMGPPSAVAERPKISLHFDTETDGYFSDGEMSDSDVEAEDGGVQRGPREAGAEEVVRMGVLAS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NQC1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PHF15 also known as Jade-1 is a protein of approximately 62 kilodaltons and is involved in chromatin remodeling. PHF15 contains a plant homeodomain (PHD) finger that likely contributes to its function in recognizing methylated histones. The protein is mainly expressed in the nucleus of kidney cells but can also be detected in other tissues. PHF15 has an important role in regulating gene expression by influencing the chromatin structure availability for transcriptional machinery.
Biological function summary

The role of PHF15 involves the regulation of gene expression during critical cellular processes. It interacts within the chromatin remodeling complex facilitating changes in the chromatin structure that are necessary for transcriptional activation or repression. The modulation of chromatin architecture by PHF15 impacts various biological activities that are essential for maintaining cell identity and function. Its precise mechanistic involvement in chromatin modifications highlights its biological importance.

Pathways

The activity of PHF15 features in the Wnt signaling pathway and the regulation of the cell cycle. These pathways involve interactions with other proteins like β-catenin and cyclin D1 where PHF15 exerts regulatory effects. In the Wnt signaling pathway PHF15 modulates gene expression impacting various cellular outcomes such as proliferation and differentiation. Through its integration in these pathways PHF15 has a fundamental influence on cellular fate and behavior.

Defects in PHF15 function connect to clear cell renal cell carcinoma. Alterations in its interaction with proteins such as von Hippel-Lindau (VHL) can disrupt normal cellular regulation contributing to disease progression. Furthermore PHF15 interacts with PBRM1 and changes in their regulation can play a role in tumorigenesis. Understanding the role of PHF15 in these contexts is significant for unraveling its contributions to disease mechanisms and potential therapeutic strategies.
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일반 정보

기능

Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed : 16387653). Acts as an E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed : 25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).

서열 유사성

Belongs to the JADE family.

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제품 프로토콜

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타겟 정보

Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed : 16387653). Acts as an E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed : 25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).
See full target information JADE2
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Product promise

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