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AB323127

Recombinant Human Phosphoserine Aminotransferase Protein Standard (His tag)

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Recombinant Human Phosphoserine Aminotransferase Protein Standard (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human Phosphoserine Aminotransferase Protein Standard (His tag) (AB323127)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Phosphoserine Aminotransferase Protein Standard (His tag) (AB323127)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Phosphoserine Aminotransferase Protein Standard (His tag) (AB323127)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Phosphoserine Aminotransferase Protein Standard (His tag) (AB323127)

SDS-PAGE analysis of ab323127 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

Q9Y617-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 12.6% Glycerol (glycerin, glycerine), 2.922% Sodium chloride, 1.742% L-Arginine, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Proteins & Peptides

AB48755

Recombinant Human PSPH protein (Tag Free)

  • 0
  • 0
Assay Kits

AB273328

Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)

Functional Studies - Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (AB273328)

  • 0
  • 0
Assay Kits

AB115348

Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit

Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

  • 0
  • 0
Primary Antibodies

AB308512

Anti-Phosphoserine Aminotransferase antibody [EPR26994-48]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody [EPR26994-48] (AB308512)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"42.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y617","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine (PubMed : 36851825, PubMed : 37627284). Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate (PubMed : 36851825, PubMed : 37627284). Acts as an inhibitor of ferroptosis in response to interferon-gamma (IFNG) by promoting GPX4 stability : following phosphorylation by CAMK2A, PSAT1 interacts with GPX4 and provides 2-oxoglutarate to EGLN3, leading to GPX4 hydroxylation and stability (PubMed : 40281343).

서열 유사성

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Post-translational modifications

Phosphorylated at Ser-337 by CAMK2A in response to interferon-gamma (IFNG), promoting interaction with GPX4 and GPX4 hydroxylation by EGLN3.

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제품 프로토콜

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타겟 정보

Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine (PubMed : 36851825, PubMed : 37627284). Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate (PubMed : 36851825, PubMed : 37627284). Acts as an inhibitor of ferroptosis in response to interferon-gamma (IFNG) by promoting GPX4 stability : following phosphorylation by CAMK2A, PSAT1 interacts with GPX4 and provides 2-oxoglutarate to EGLN3, leading to GPX4 hydroxylation and stability (PubMed : 40281343).
See full target information PSAT1

대체 명칭 보기

PSA, Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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