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AB176047

Recombinant Human PIH1D1 protein (denatured) (His tag N-Terminus)

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Recombinant Human PIH1D1 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 290 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

NOP17, PIH1D1, PIH1 domain-containing protein 1, Nucleolar protein 17 homolog

1 이미지
SDS-PAGE - Recombinant Human PIH1D1 protein (denatured) (His tag N-Terminus) (AB176047)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PIH1D1 protein (denatured) (His tag N-Terminus) (AB176047)

15% SDS-PAGE analysis of ab176047 (3μg).

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer

storage-buffer

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보완 제품

Primary Antibodies

AB238862

Anti-PIH1D1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIH1D1 antibody (AB238862)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMANPKLLGMGLSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEVDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLPVPS","proteinLength":"Full Length","predictedMolecularWeight":"34.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":290,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NWS0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PIH1D1 also known as PIH1 domain containing protein 1 plays a mechanical role in stabilizing multi-protein complexes. It weighs approximately 37 kDa. The protein exists mainly within the cytoplasm and nucleus of cells. PIH1D1 is a critical component of the R2TP complex contributing to its structural integrity and function. The R2TP complex plays a significant role in various cellular processes including transcription and the assembly of complexes like snoRNPs.
Biological function summary

PIH1D1 interacts with other proteins to mediate molecular chaperone activities. It forms part of the R2TP complex which stands for RNA polymerase II-associated protein 2; TP is related to tuberous sclerosis complexes. Together with other proteins such as RPAP3 it assists in assembling large multi-subunit complexes. This function is essential in maintaining normal cellular operations and stress responses facilitating the maturation and stabilization of many other proteins.

Pathways

PIH1D1 involves itself in the molecular chaperone network and mTOR signaling pathway. It interacts primarily with proteins related to the chaperone HSP90 where it helps to fold and stabilize newly synthesized proteins. The contact with mTORC1 complex which connects to growth and metabolic pathways illustrates its significant contributions to cell growth regulation and energy status sensing. PIH1D1’s role ensures that the pathways remain functional and adaptable.

Researchers have linked PIH1D1 to certain types of cancer and neurodevelopmental disorders. Its interaction with the tumor suppressor protein TSC1 suggests relevance in cancer pathogenesis where different mutations can disrupt normal cell growth control. Moreover piH1D1's involvement in the assembly of snoRNPs connects it to neurodevelopmental disorders where correct RNA processing is essential. Changes in its function can lead to significant cellular abnormalities contributing to disease progression.
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일반 정보

기능

Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed : 17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed : 22368283, PubMed : 24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed : 20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed : 24036451).

서열 유사성

Belongs to the PIH1 family.

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제품 프로토콜

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타겟 정보

Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed : 17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed : 22368283, PubMed : 24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed : 20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed : 24036451).
See full target information PIH1D1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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