AB60850

Recombinant human PKC iota protein (GST tag N-Terminus)

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Recombinant human PKC iota protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

12 이미지

Functional Studies - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

FuncS

Unknown

Functional Studies - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

The specific activity of PKC iota (ab60850) was determined to be 572 nmol/min/mg as per activity assay protocol and was equivalent to 564 nmol/min/mg as per radiometric assay

FuncS

Unknown

Functional Studies - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Sample Kinase Activity Plot.

Unknown

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

All lanes:

Anti-PKC iota antibody (<a href='/ko/products/unavailable/pkc-iota-antibody-ab5282'>ab5282</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850) at 0.1 µg

Lane 1:

Recombinant rat MCP1 protein (<a href='/ko/products/unavailable/recombinant-rat-mcp1-protein-ab9780'>ab9780</a>) at 1/5000 dilution

true

Exposure time: 1min

Supplier Data

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

5% NFDM/TBST All positive controls 10 ng

All lanes:

Western blot - Anti-PKC alpha antibody [133] (<a href='/ko/products/primary-antibodies/pkc-alpha-antibody-133-ab11723'>ab11723</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 5:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 6:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 7:

Western blot - Recombinant human PKC mu/PKD protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>)

Lane 8:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850)

Lane 10:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false

Supplier Data

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62372).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (<a href='/ko/products/primary-antibodies/pkc-zeta-phospho-t560-antibody-ep2037ay-ab62372'>ab62372</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 105 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

This data was developed using ab76129, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 180 seconds; Lane 5-6 : 60 seconds; Lane 7-10 : 5 seconds.

All lanes:

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (<a href='/ko/products/primary-antibodies/pkc-zeta-phospho-t410-pkc-iota-phospho-t412-antibody-ep1491y-ab76129'>ab76129</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa

false

SDS-PAGE - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

SDS PAGE analysis of ab60850

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

ab60850 on SDS-PAGE, MW ~98 kDa.

Supplier Data

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32376).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha antibody [Y124] (<a href='/ko/products/primary-antibodies/pkc-alpha-antibody-y124-ab32376'>ab32376</a>) at 1/2000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 20s

Supplier Data

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

This data was developed using ab32502, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha (phospho T638) antibody [E195] (<a href='/ko/products/primary-antibodies/pkc-alpha-phospho-t638-antibody-e195-ab32502'>ab32502</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 7:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 8:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 60s

Supplier Data

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

This data was developed using ab53295, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (<a href='/ko/products/primary-antibodies/pkc-delta-phospho-t507-antibody-epr1485y-ab53295'>ab53295</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 7:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 8:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 105 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

This data was developed using ab180848, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (<a href='/ko/products/primary-antibodies/pkc-alpha-phospho-s657-antibody-epr19012-ab180848'>ab180848</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (ab60850)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 60s

주요 정보

Purity

>90% Densitometry

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

P41743-1

Animal free

Carrier free

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab60850 can be used as a WB positive control in conjunction with <a href='/ko/products/unavailable/pkc-iota-antibody-ab5282'>ab5282</a>." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Kinase assay. (664 nmol/min/mg)." } } }

style

left-column

제품 세부 정보

ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

style

left-column

서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P41743","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

True

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Protein Kinase C (PKC) iota also known by its gene symbol PRKCI is an atypical member of the PKC family. It has a molecular mass of approximately 74 kDa. PKC iota does not respond to calcium and is insensitive to diacylglycerol and phorbol esters which differentiates it from other classical PKC family members. The protein initiates cell survival and proliferation pathways and is primarily expressed in brain heart liver and skeletal muscle tissues indicating its extensive role across various biological functions.

Biological function summary

PKC iota acts as an important player in regulating cell polarity and protecting cells from stress-induced apoptosis. It forms part of the Par6-Par3-PKC iota complex which is important for maintaining cell polarity and directional migration. This complex is essential for neural tube closure during embryonic development and in establishing cell polarity across diverse cell types. PKC iota also contributes to the regulation of microtubule organization reinforcing its vital role in maintaining cellular architecture.

Pathways

PKC iota is important in the NF-kB signaling pathway and the PI3K/AKT pathway. In the NF-kB pathway PKC iota activates IkB kinase leading to NF-kB transcriptional activity which promotes cell survival. In the PI3K/AKT pathway PKC iota contributes to cell growth and survival working closely with AKT proteins. These pathways highlight its involvement in processes like proliferation survival and cellular response to external stimuli.

PKC iota has a significant connection to cancer and neurological disorders. Its overexpression has been observed in several cancers such as lung and prostate cancer where it is often associated with poor prognosis. PKC iota’s role in the maintenance of cell polarity and survival links it to malignant cell behavior. Additionally in neurological disorders like Alzheimer’s disease alterations in PKC iota activity have been related to synaptic dysfunctions. Connections to proteins such as AKT and NF-kB in these conditions highlight its potential as a therapeutic target.

style

left-column

일반 정보

기능

Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Involved in early synaptic long term potentiation phase in CA1 hippocampal cells and short term memory formation (By similarity).

서열 유사성

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Post-translational modifications

Phosphorylation at Thr-412 in the activation loop is not mandatory for activation (By similarity). Upon neuronal growth factor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr-280 and Tyr-334 (PubMed:11713277, PubMed:16452474). Phosphorylation at Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus (PubMed:11891849). Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation (PubMed:11713277). Phosphorylated at Thr-564 during the initial phase of long term potentiation (By similarity).

Subcellular localisation

Endosome

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제품 프로토콜

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타겟 정보

See full target information PRKCI

대체 명칭 보기

DXS1179E, PRKCI, Protein kinase C iota type, Atypical protein kinase C-lambda/iota, nPKC-iota, PRKC-lambda/iota, aPKC-lambda/iota

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Product promise

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Recombinant human PKC iota protein (GST tag N-Terminus)

Functional Studies - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Functional Studies - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

SDS-PAGE - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

SDS-PAGE - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (AB60850)

주요 정보

Purity

발현 시스템

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

보관 버퍼

보완 제품

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Reactivity 정보

제품 세부 정보

서열 정보

특성 및 보관 정보

제형

Purification 테크닉

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

일반 정보

기능

서열 유사성

Post-translational modifications

Subcellular localisation

제품 프로토콜

타겟 정보

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