AB60848

Recombinant human PKC zeta protein (GST tag N-Terminus)

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Recombinant human PKC zeta protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

12 이미지

Functional Studies - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

FuncS

Unknown

Functional Studies - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Sample Kinase Activity Plot.

FuncS

Unknown

Functional Studies - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

The specific activity of PKC zeta (ab60848) was determined to be 103 nmol/min/mg as per activity assay protocol

Unknown

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

All lanes:

Anti-PKC zeta antibody (<a href='/ko/products/unavailable/pkc-zeta-antibody-ab51157'>ab51157</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

true

Exposure time: 1min

Supplier Data

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

5% NFDM/TBST All positive controls 10 ng

All lanes:

Western blot - Anti-PKC alpha antibody [133] (<a href='/ko/products/primary-antibodies/pkc-alpha-antibody-133-ab11723'>ab11723</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 5:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 6:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 7:

Western blot - Recombinant human PKC mu/PKD protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>)

Lane 8:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848)

Lane 9:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Lane 10:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false

Supplier Data

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62372).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (<a href='/ko/products/primary-antibodies/pkc-zeta-phospho-t560-antibody-ep2037ay-ab62372'>ab62372</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 105 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

This data was developed using ab76129, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 180 seconds; Lane 5-6 : 60 seconds; Lane 7-10 : 5 seconds.

All lanes:

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (<a href='/ko/products/primary-antibodies/pkc-zeta-phospho-t410-pkc-iota-phospho-t412-antibody-ep1491y-ab76129'>ab76129</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa

false

SDS-PAGE - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

ab60848 on SDS-PAGE, MW ~93 kDa.

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

SDS PAGE analysis of ab60848

Supplier Data

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32376).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha antibody [Y124] (<a href='/ko/products/primary-antibodies/pkc-alpha-antibody-y124-ab32376'>ab32376</a>) at 1/2000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 20s

Supplier Data

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

This data was developed using ab32502, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha (phospho T638) antibody [E195] (<a href='/ko/products/primary-antibodies/pkc-alpha-phospho-t638-antibody-e195-ab32502'>ab32502</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848)

Lane 7:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 8:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 60s

Supplier Data

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

This data was developed using ab53295, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (<a href='/ko/products/primary-antibodies/pkc-delta-phospho-t507-antibody-epr1485y-ab53295'>ab53295</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848)

Lane 7:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 8:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 105 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

This data was developed using ab180848, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (<a href='/ko/products/primary-antibodies/pkc-alpha-phospho-s657-antibody-epr19012-ab180848'>ab180848</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (ab60848)

Lane 8:

Western blot - Recombinant human PKC eta protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (GST tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 105 kDa

false

Exposure time: 60s

주요 정보

Purity

>90% SDS-PAGE

Purity: >90% as determined by densitometry. Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

Q05513-1

Animal free

Carrier free

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab60848 can be used as a WB positive control in conjunction with <a href='/ko/products/unavailable/pkc-zeta-antibody-ab51157'>ab51157</a>." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Specific Activity: 114 nmol/min/mg." } } }

style

left-column

제품 세부 정보

ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

style

left-column

서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q05513","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

True

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PKC zeta also known as Protein Kinase C zeta is a member of the atypical protein kinase C family. This enzyme plays an important role in various cellular processes. PKC zeta has a molecular mass of about 55 kDa and is expressed in many tissues including brain and immune cells. Unlike conventional PKC enzymes PKC zeta does not respond to calcium or diacylglycerol which influences its unique regulatory roles.

Biological function summary

PKC zeta participates in regulating cell proliferation survival and differentiation. It often functions as part of signaling complexes that include other proteins like PDK1 and is involved in transducing signals from cell surface receptors to the nucleus. Its activity influences cellular responses to hormones growth factors and cytokines contributing to diverse cellular events.

Pathways

PKC zeta plays a critical role in the NF-kappaB and PI3K/Akt signaling pathways. In the NF-kappaB pathway PKC zeta helps in the activation of this transcription factor affecting genes related to inflammation and immune response. In the PI3K/Akt pathway it is involved in cell growth and survival interacting with kinases such as Akt and PDK1 which further modulate downstream effectors.

PKC zeta has associations with cancer and insulin resistance-related conditions. In cancer dysregulation of PKC zeta can lead to unchecked cellular proliferation due to its involvement in survival pathways and it often interacts with oncogenic proteins like Akt. In insulin resistance PKC zeta misregulation impacts glucose metabolism highlighting its role in diabetes-related pathways alongside insulin receptor substrates.

style

left-column

일반 정보

기능

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed : 17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).. Isoform 2. Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.

서열 유사성

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Post-translational modifications

CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.

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See full target information PRKCZ

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PKC2, PRKCZ, Protein kinase C zeta type, nPKC-zeta

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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70528 PubMed40193069

2025

Intracellular Sphingosine-1-Phosphate Induces Lipolysis Through Direct Activation of Protein Kinase C Zeta.

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Sarah Weske,Melissa Kim Nowak,Alex Zaufel,Lea Esser,Christoph Peter,Lisa Walz,Helena Kühn,Tsyon Wolde,Julia Hoppe,Nathalie Hannelore Schröder,Tobias Buschmann,Philipp Wollnitzke,Bodo Levkau

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Recombinant human PKC zeta protein (GST tag N-Terminus)

Functional Studies - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Functional Studies - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

SDS-PAGE - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

SDS-PAGE - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

Western blot - Recombinant human PKC zeta protein (GST tag N-Terminus) (AB60848)

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