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AB109945

Recombinant Human PME-1 protein (His tag N-Terminus)

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Recombinant Human PME-1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 386 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

PME1, PP2593, PRO0750, PPME1, Protein phosphatase methylesterase 1, PME-1

1 이미지
SDS-PAGE - Recombinant Human PME-1 protein (His tag N-Terminus) (AB109945)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PME-1 protein (His tag N-Terminus) (AB109945)

15% SDS-PAGE analysis of 3μg ab109945.

주요 정보

Purity

>95% SDS-PAGE

ab109945 was purified using conventional chromatography.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.058% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Proteins & Peptides

AB128551

Recombinant human PPP1CB

Phosphatase Activity - Recombinant human PPP1CB (AB128551)

  • 0
  • 0
Primary Antibodies

AB154569

Anti-PME-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PME-1 antibody (AB154569)

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  • 0
Proteins & Peptides

AB104668

Recombinant Human hnRNP C1/C2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human hnRNP C1/C2 protein (His tag N-Terminus) (AB104668)

  • 0
  • 0
Proteins & Peptides

AB186079

Recombinant Human TRIB1 protein

SDS-PAGE - Recombinant Human TRIB1 protein (AB186079)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Previously labelled as PPME1.
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC","proteinLength":"Full Length","predictedMolecularWeight":"44.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":386,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y570","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase methylesterase 1 (PME-1) also known as protein phosphatase 2A methylesterase is an enzyme that regulates protein function by demethylation. PME-1 has a mass of approximately 42 kDa. It primarily resides in the cytoplasm and nucleus of cells with high expression levels in brain tissue. Its function involves the removal of methyl groups from the carboxyl-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (PP2A). This action regulates the activity states of PP2A a major serine/threonine phosphatase.
Biological function summary

PME-1 influences the stability and activity of PP2A a core component of cellular signal transduction pathways. PME-1 forms part of the regulatory complexes of PP2A modulating its methylation state and associated functional properties. This regulation by PME-1 impacts cell cycle control apoptosis and other critical cellular processes. Additionally PME-1 activity affects signaling pathways by altering PP2A's interaction with various substrates and regulatory proteins.

Pathways

The role of PME-1 in cellular mechanisms links it to the Wnt signaling pathway and MAP kinase signaling cascade. PME-1 catalyzes demethylation reactions that impact PP2A's ability to associate with specific regulatory subunits. Through the Wnt pathway PME-1 affects cellular processes such as proliferation and differentiation by regulating beta-catenin activity. In relation to the MAP kinase pathway PME-1 influences cell stress response and growth factor signaling interacting with proteins like ERK and p38 MAP kinase.

PME-1 has associations with cancer and neurodegenerative conditions. In cancer altered PME-1 expression or activity can lead to uncontrolled cell growth and survival acting through PP2A-related pathways. In neurodegenerative diseases such as Alzheimer's PME-1 may affect tau protein phosphorylation by modulating PP2A activity potentially contributing to pathogenesis. As PP2A regulates tau phosphorylation disruptions in its demethylation by PME-1 has been correlated with disease progression.
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일반 정보

기능

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.

서열 유사성

Belongs to the AB hydrolase superfamily.

Post-translational modifications

Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.

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제품 프로토콜

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타겟 정보

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.
See full target information PPME1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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