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AB99391

Recombinant Human PMM2 protein (His tag N-Terminus)

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Recombinant Human PMM2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
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SDS-PAGE - Recombinant Human PMM2 protein (His tag N-Terminus) (AB99391)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PMM2 protein (His tag N-Terminus) (AB99391)

15% SDS-PAGE analysis of 3μg ab99391.

주요 정보

Purity

>90% SDS-PAGE

ab99391 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O15305-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

  • 0
  • 0
Primary Antibodies

AB229996

Anti-PMM2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

  • 0
  • 0
Primary Antibodies

AB96193

Anti-MDH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (AB96193)

  • 5
  • 2
Primary Antibodies

AB174288

Anti-DHODH antibody [EPR11814]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-DHODH antibody [EPR11814] (AB174288)

  • 0
  • 0
Primary Antibodies

AB32441

Anti-SIRT1 antibody [E104]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SIRT1 antibody [E104] (AB32441)

  • 4
  • 13
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMAAPGPALCLFDVDGTLTAPRQKITKEMDDFLQKLRQKIKIGVVGGSDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCRQNIQSHLGEALIQDLINYCLSYIAKIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKENIRQKFVADLRKEFAGKGLTFSIGGQISFDVFPDGWDKRYCLRHVENDGYKTIYFFGDKTMPGGNDHEIFTDPRTMGYSVTAPEDTRRICELLFS","proteinLength":"Full Length","predictedMolecularWeight":"30.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15305","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomannomutase 2 (PMM2) also known as PMM 22 or PMM II is an enzyme that plays a role in the conversion of mannose-6-phosphate to mannose-1-phosphate. PMM2 is about 29 kDa in size and exists predominantly in the cytoplasm of cells. It shows widespread expression but higher levels are found in the liver brain and muscle tissues. PMM2 activity is important in carbohydrate metabolism making it an enzyme of interest especially in the context of glycobiology and metabolic research.
Biological function summary

PMM2 catalyzes an important step in the biosynthesis of GDP-mannose which is necessary for glycan production. These glycans are essential in the process of glycosylation a post-translational modification of proteins and lipids. PMM2 acts independently and does not form part of a larger protein complex. Through its role in glycan synthesis PMM2 impacts cell signaling protein folding and molecular interactions.

Pathways

PMM2 is involved in the N-glycosylation pathway and the GDP-mannose biosynthesis pathway. It collaborates closely with proteins such as PMM1 as both contribute to mannose metabolism and glycan assembly. PMM2 is significant in these pathways as it impacts not only the formation of sugar nucleotides but also their integration into larger biomolecules.

PMM2 dysfunction is directly linked to Congenital Disorders of Glycosylation Type Ia (CDG-Ia). This genetic disorder results from mutations in the PMM2 gene impairing glycosylation processes and resulting in multisystemic clinical manifestations. The protein PMM1 while not directly associated with the disorder serves complementary functions in mannose phosphate isomerization and may modulate the impact of PMM2 deficiencies.
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일반 정보

기능

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

서열 유사성

Belongs to the eukaryotic PMM family.

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제품 프로토콜

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타겟 정보

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
See full target information PMM2

대체 명칭 보기

Phosphomannomutase 2, PMM 2, PMM2
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Product promise

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