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AB101213

Recombinant Human PNPO protein (His tag N-Terminus)

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Recombinant Human PNPO protein (His tag N-Terminus) is a Human Fragment protein, in the 57 to 261 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

Pyridoxine-5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, PNPO

1 이미지
SDS-PAGE - Recombinant Human PNPO protein (His tag N-Terminus) (AB101213)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PNPO protein (His tag N-Terminus) (AB101213)

SDS-PAGE of ab101213 (3µg) under reducing condition and visualized by coomassie blue stain.

주요 정보

Purity

>95% SDS-PAGE

ab101213 was purified using conventional chromatography.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.00174% PMSF

storage-buffer

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보완 제품

Primary Antibodies

AB288067

Anti-TPH2 antibody [EPR25100-29]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-TPH2 antibody [EPR25100-29] (AB288067)

  • 0
  • 0
Primary Antibodies

AB203745

Anti-PNPO antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPO antibody (AB203745)

  • 0
  • 0
Primary Antibodies

AB6336

Anti-Serotonin antibody [YC5/45]

BOND RX™ Validated

Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (AB6336)

  • 5
  • 3
Primary Antibodies

AB66047

Anti-Serotonin antibody

Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody (AB66047)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMDPVKQFAAWFEEAVQCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP","proteinLength":"Fragment","predictedMolecularWeight":"25.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":261,"aminoAcidStart":57,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NVS9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The PNPO protein also known as pyridoxine 5'-phosphate oxidase plays a mechanical role in the biosynthesis of pyridoxal 5'-phosphate (PLP) the active form of vitamin B6. This enzyme with a mass of approximately 30 kDa catalyzes the oxidation of pyridoxine 5'-phosphate (PNP) to PLP. PNPO is expressed widely in human tissues particularly in the liver kidney and brain where vitamin B6 metabolism is essential.
Biological function summary

PNPO contributes to the production of PLP a cofactor involved in numerous enzymatic reactions including neurotransmitter synthesis and amino acid metabolism. PLP acts as a critical helper molecule in the functioning of over 140 enzymes. PNPO operates as part of a multi-step enzyme complex responsible for the conversion of dietary vitamin B6 forms into PLP ensuring the availability of this vital coenzyme throughout the body.

Pathways

PNPO integrates into the vitamin B6 metabolism pathway which is important for maintaining cellular functions. This enzyme assures the proper transformation of PNP to PLP coordinating with other enzymes such as pyridoxal kinase to regulate levels of active vitamin B6. PNPO's activity influences pathways like the transsulfuration and tryptophan degradation which rely on PLP-dependent enzymes.

PNPO deficiencies can lead to neurological conditions such as epilepsy due to impaired PLP production which affects neurotransmitter synthesis. Mutations in the PNPO gene may result in an inborn error of metabolism. In some cases PNPO interacts with other proteins such as glutamic acid decarboxylase which depend on PLP for activity. These interactions can exacerbate disease phenotypes highlighting PNPO's importance in maintaining neurological health.
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일반 정보

기능

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).

서열 유사성

Belongs to the pyridoxamine 5'-phosphate oxidase family.

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제품 프로토콜

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타겟 정보

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).
See full target information PNPO
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Product promise

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