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AB202165

Recombinant Human PRD protein (His tag N-Terminus)

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Recombinant Human PRD protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 493 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

PRD, PEPD, Xaa-Pro dipeptidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D, Proline dipeptidase, Prolidase

1 이미지
SDS-PAGE - Recombinant Human PRD protein (His tag N-Terminus) (AB202165)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PRD protein (His tag N-Terminus) (AB202165)

15% SDS-PAGE analysis of 3 μg ab202165.

주요 정보

Purity

>90% SDS-PAGE

ab202165 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB197890

Anti-PRD antibody [EPR16959]

RabMAb|Recombinant

Western blot - Anti-PRD antibody [EPR16959] (AB197890)

  • 0
  • 0
Proteins & Peptides

AB316535

Recombinant VILIP1 Protein Standard (His tag)

Sandwich ELISA - Recombinant VILIP1 Protein Standard (His tag) (AB316535)

  • 0
  • 0
Primary Antibodies

AB78294

Anti-LTBP1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (AB78294)

  • 5
  • 1
Primary Antibodies

AB202445

Anti-Smad2 + Smad3 antibody [EPR19557-4] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Smad2 + Smad3 antibody [EPR19557-4] - ChIP Grade (AB202445)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as PEPD
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK","proteinLength":"Full Length","predictedMolecularWeight":"56.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":493,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P12955","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PRD also known as the podocyte retinoid-dependent domain is a protein of interest in several research areas. This protein has a known mass of approximately 62 kDa. PRD expresses in kidney podocytes specialized cells responsible for filtration in the renal glomerulus. The protein plays an important role mechanically in maintaining podocyte structure and function contributing to the integrity of the glomerular filtration barrier.
Biological function summary

PRD interacts with cellular mechanisms critical for podocyte stability. PRD associates with lipid rafts specialized membrane structures suggesting a role in signal transduction. It functions as part of a larger protein complex that includes nephrin and podocin essential components of the slit diaphragm in podocytes. PRD is involved in cytoskeletal dynamics regulating actin filament organization important for maintaining cell shape and adhesion.

Pathways

Several cellular activities incorporate PRD into their processes. It is closely tied to the PI3K/AKT signaling pathway a critical pathway regulating cell survival and growth. In the slit diaphragm context PRD works alongside nephrin and p130Cas forming a signaling cascade that ensures effective communication between podocytes. These pathways help maintain the health of the glomerular filtration system by responding to mechanical and biochemical stimuli.

The disruption of PRD function links to renal diseases such as nephrotic syndrome and focal segmental glomerulosclerosis (FSGS). Mutations or alterations in PRD expression or interaction can impair the slit diaphragm leading to proteinuria and progressive kidney damage. The protein interacts with nephrin and podocin whose mutations are also implicated in these disorders highlighting its importance in preserving kidney function.
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일반 정보

기능

Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position (PubMed : 17081196, PubMed : 35165443). The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved (PubMed : 17081196). Plays an important role in collagen metabolism because the high level of iminoacids in collagen (PubMed : 2925654).

서열 유사성

Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.

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제품 프로토콜

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타겟 정보

Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position (PubMed : 17081196, PubMed : 35165443). The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved (PubMed : 17081196). Plays an important role in collagen metabolism because the high level of iminoacids in collagen (PubMed : 2925654).
See full target information PEPD
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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