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AB115714

Recombinant Human PSME3 protein (His tag N-Terminus)

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Recombinant Human PSME3 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 254 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

Proteasome activator complex subunit 3, 11S regulator complex subunit gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, Proteasome activator 28 subunit gamma, REG-gamma, PA28g, PA28gamma, PSME3

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SDS-PAGE - Recombinant Human PSME3 protein (His tag N-Terminus) (AB115714)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PSME3 protein (His tag N-Terminus) (AB115714)

SDS-PAGE analysis of ab115714 (3µg).

주요 정보

Purity

>90% SDS-PAGE

ab115714 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB157157

Anti-PSME3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody (AB157157)

  • 5
  • 2
Proteins & Peptides

AB206448

Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (His tag N-Terminus) (AB206448)

  • 0
  • 0
Proteins & Peptides

AB164415

Recombinant Human TEX101 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human TEX101 protein (GST tag N-Terminus) (AB164415)

  • 0
  • 0
Proteins & Peptides

AB153778

Recombinant Human Cysteine Dioxygenase CDO1 protein

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMASLLKVDQEVKLKVDSFRERITSEAEDLVANFFPKKLLELDSFLKEPILNIHDLTQIHSDMNLPVPDPILLTNSHDGLDGPTYKKRRLDECEEAFQGTKVFVMPNGMLKSNQQLVDIIEKVKPEIRLLIEKCNTVKMWVQLLIPRIEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITRAKLVSKIAKYPHVEDYRRTVTEIDEKEYISLRLIISELRNQYVTLHDMILKNIEKIKRPRSSNAETLY","proteinLength":"Full Length","predictedMolecularWeight":"31.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":254,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61289","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The PSME3 protein also known as PA28γ or REGγ is mechanically an essential proteasome activator that facilitates the degradation of ubiquitin-independent substrates. It has a molecular weight of approximately 29 kDa. PSME3 is mainly found in the nucleus and shows strong expression in various tissues including liver kidney and heart. The protein operates by binding to the 20S proteasome core and enhances the specificity of proteolytic activity.
Biological function summary

PSME3 regulates cell cycle progression and apoptosis. It is part of a larger multiprotein complex with the 20S proteasome core forming an important component in protein homeostasis. PSME3’s interaction with the proteasome core significantly reduces the accumulation of certain regulatory proteins by promoting their degradation. This function affects several cellular processes such as cell proliferation and DNA repair aiding the maintenance of cellular function and integrity.

Pathways

PSME3 plays a critical role in mediating the ubiquitin-proteasome pathway and the MDM2-p53 pathway. In the ubiquitin-proteasome pathway it assists in the controlled turnover of intracellular proteins which is pivotal for maintaining cellular protein quality. In the MDM2-p53 pathway it affects the stability of the p53 protein by enhancing the degradation of both MDM2 and p53 therefore modulating apoptosis and growth arrest in cells under stress or DNA damage conditions.

Disruptions in PSME3 function correlate with cancer and neurodegenerative disorders. Overexpression of PSME3 is often linked to oncogenesis due to its role in controlling the degradation of tumor suppressor proteins like p53. This protein is also implicated in Parkinson’s disease where its interaction with pathogenic proteins suggests it might contribute to the accumulation of misfolded proteins. PSME3 along with its pathways and related proteins offers potential as a target for therapeutic intervention in these diseases.
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일반 정보

기능

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed : 25361978).

서열 유사성

Belongs to the PA28 family.

Post-translational modifications

Phosphorylated by MAP3K3 (By similarity). Phosphorylation at Ser-247 promotes its association with CCAR2.. Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed : 25361978).
See full target information PSME3
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Product promise

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