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AB101948

Recombinant Human PYCR1 protein (His tag N-Terminus)

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Recombinant Human PYCR1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 319 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, FuncS.

대체 명칭 보기

P5C reductase 1, P5CR 1, PYCR1

1 이미지
SDS-PAGE - Recombinant Human PYCR1 protein (His tag N-Terminus) (AB101948)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PYCR1 protein (His tag N-Terminus) (AB101948)

15% SDS-PAGE analysis of 3μg ab101948.

주요 정보

Purity

>90% SDS-PAGE

ab101948 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, Mass Spec, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

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  • 1
Primary Antibodies

AB279385

Anti-PYCR1 antibody [OTI4F2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

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Proteins & Peptides

AB159577

Recombinant Human SREBP1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human SREBP1 protein (GST tag N-Terminus) (AB159577)

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  • 0
Proteins & Peptides

AB79185

Recombinant Human Myosin Light Chain 2 protein (His tag N-Terminus)

Western blot - Recombinant Human Myosin Light Chain 2 protein (His tag N-Terminus) (AB79185)

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  • 0
Proteins & Peptides

AB176044

Recombinant Human VAV1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human VAV1 protein (His tag N-Terminus) (AB176044)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPAAIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD","proteinLength":"Full Length","predictedMolecularWeight":"35.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":319,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P32322","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PYCR1 also known as Pyrroline-5-carboxylate reductase 1 is an enzyme with a molecular weight of approximately 33 kDa. It facilitates the conversion of pyrroline-5-carboxylate to proline an essential amino acid. PYCR1 is mainly expressed in human tissues with high metabolic activity such as the liver kidney and pancreas which highlights its role in amino acid metabolism. This enzyme plays a central role in maintaining the balance between cellular oxidation and reduction and is typically active in the cytoplasm of the cells.
Biological function summary

PYCR1 participates in the proline biosynthesis pathway and contributes to the cellular response to stress. It functions as a homopolymer indicating its need to form complexes for activity. The production of proline is important as proline protects cells by maintaining membrane integrity and stabilizing proteins during environmental stress like oxidative stress. Proline synthesis involving PYCR1 is essential for collagen production which supports tissue and structural integrity in humans.

Pathways

PYCR1 acts within the proline biosynthesis pathway and the larger metabolism of amino acids. This enzyme works closely with ALDH4A1 which is another enzyme that catalyzes the earlier stages of the proline biosynthesis pathway before PYCR1 performs its function. The pathway plays a significant role in plant and animal cell adaptation to environmental changes. By modulating these pathways cells gain enhanced abilities to manage osmotic pressures and support vital functions under stress conditions linking PYCR1's activity to cellular survival and resilience.

PYCR1 mutations or dysfunction is associated with Cutis laxa a rare connective tissue disorder characterized by loose sagging skin and systemic involvement. These dysfunctions affect proline synthesis impairing collagen production which leads to abnormal connective tissue structure. PYCR1 is also connected to pyrroline-5-carboxylate synthetase implicating dysfunction in the larger metabolic framework that supports protein synthesis and cellular health. These disturbances underline the enzyme's importance in maintaining normal physiological conditions and hint at possible therapeutic targets for related disorders.
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일반 정보

기능

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 16730026, PubMed : 19648921, PubMed : 23024808, PubMed : 28258219). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 16730026, PubMed : 23024808). Involved in the cellular response to oxidative stress (PubMed : 16730026, PubMed : 19648921).

서열 유사성

Belongs to the pyrroline-5-carboxylate reductase family.

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제품 프로토콜

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타겟 정보

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 16730026, PubMed : 19648921, PubMed : 23024808, PubMed : 28258219). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 16730026, PubMed : 23024808). Involved in the cellular response to oxidative stress (PubMed : 16730026, PubMed : 19648921).
See full target information Pyrroline-5-carboxylate reductase 1, mitochondrial
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Product promise

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