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AB139626

Recombinant Human PYCRL protein (His tag N-Terminus)

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(1 리뷰)

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(0 출판물)

Recombinant Human PYCRL protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 274 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

PYCRL, PYCR3, Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase-like protein

2 이미지
SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)

SDS-PAGE analysis of ab139626

SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)

15% SDS-PAGE analysis of ab139626 (3μg).

주요 정보

Purity

>90% SDS-PAGE

ab139626 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Secondary Antibodies

AB96873

Goat Anti-Mouse IgG H&L (DyLight® 594)

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  • 1
Proteins & Peptides

AB135223

Recombinant Human INCENP protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human INCENP protein (AB135223)

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  • 0
Proteins & Peptides

AB236924

Recombinant Mouse Carboxylesterase 1C protein

SDS-PAGE - Recombinant Mouse Carboxylesterase 1C protein (AB236924)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK","proteinLength":"Full Length","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":274,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q53H96","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PYCRL also known as Pyrroline-5-carboxylate reductase 3 is an enzyme involved in proline biosynthesis. It catalyzes the conversion of pyrroline-5-carboxylate to proline. This protein has a molecular mass of approximately 29 kDa. It is widely expressed in various tissues including the liver kidney and placenta. Its activity plays an important role in maintaining proline homeostasis within cells.
Biological function summary

PYCRL contributes to the cellular process of amino acid metabolism. It participates in a network of enzymatic reactions that balance the levels of proline an amino acid important for protein synthesis and cellular response to osmotic stress. PYCRL forms part of a proline metabolic pathway but does not operate as part of a larger protein complex. By converting pyrroline-5-carboxylate to proline it helps in collagen synthesis and other proline-related functions within the body.

Pathways

PYCRL functionally integrates into the proline biosynthesis pathway. This pathway is essential for amino acid metabolism and stress response. PYCRL works alongside proteins such as ALDH18A1 which synthesizes pyrroline-5-carboxylate from glutamate. The proline pathway not only supports protein production but also impacts cellular stress responses by maintaining proline as an osmoprotectant.

Proline metabolism dysregulation involving PYCRL has links to certain health conditions. Alterations in this pathway can contribute to hyperprolinemia a metabolic disorder characterized by elevated proline levels. Hyperprolinemia is related to symptoms affecting neurological development. Variations in PYCRL activity might also impact connective tissue disorders due to its role in collagen production. The enzyme is potentially connected with ALDH18A1 through their shared involvement in proline metabolism influencing disease expression.
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일반 정보

기능

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate (P5C) to L-proline using NAD(P)H (PubMed : 23024808, PubMed : 36414121). Proline is synthesized from either glutamate or ornithine; both are converted to P5C, and then to proline via pyrroline-5-carboxylate reductases (PYCRs) (PubMed : 23024808). PYCR3 is exclusively linked to the biosynthesis of proline from ornithine (PubMed : 23024808).

서열 유사성

Belongs to the pyrroline-5-carboxylate reductase family.

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제품 프로토콜

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타겟 정보

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate (P5C) to L-proline using NAD(P)H (PubMed : 23024808, PubMed : 36414121). Proline is synthesized from either glutamate or ornithine; both are converted to P5C, and then to proline via pyrroline-5-carboxylate reductases (PYCRs) (PubMed : 23024808). PYCR3 is exclusively linked to the biosynthesis of proline from ornithine (PubMed : 23024808).
See full target information PYCR3
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Product promise

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