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AB188614

Recombinant Human RAD52 protein

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Recombinant Human RAD52 protein is a Human Full Length protein, in the 1 to 418 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.
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SDS-PAGE - Recombinant Human RAD52 protein (AB188614)
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Supplier Data

SDS-PAGE - Recombinant Human RAD52 protein (AB188614)

SDS-PAGE analysis of ab188614.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

Tag free

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P43351-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 6.5 - 8.5 Constituents: 20% Glycerol (glycerin, glycerine), 1.5% Potassium chloride, 0.42% Tripotassium orthophosphate, 0.02% 2-Mercaptoethanol, 0.01% EDTA

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Proteins & Peptides

AB101216

Recombinant Human RPA32/RPA2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human RPA32/RPA2 protein (His tag N-Terminus) (AB101216)

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  • 0
Assay Kits

AB139476

ROS/Superoxide Detection Assay Kit (Cell-based)

Flow Cytometry - ROS/Superoxide Detection Assay Kit (Cell-based) (AB139476)

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  • 0
Primary Antibodies

AB312914

Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

Recombinant

Flow Cytometry - Alexa Fluor® 594 Anti-CD11b antibody [M1/70] (AB312914)

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Proteins & Peptides

AB268521

Recombinant human EIF2S1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human EIF2S1 protein (His tag) (AB268521)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVESEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS","proteinLength":"Full Length","predictedMolecularWeight":"46 kDa","actualMolecularWeight":null,"aminoAcidEnd":418,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P43351","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RAD52 also known by alternate names such as DNA repair protein RAD52 homolog functions as an important player in DNA repair mechanisms. It aids in the homologous recombination and repair of DNA double-strand breaks. RAD52 has a molecular mass of approximately 49 kDa and is mainly expressed in the nucleus. Its ability to bind to DNA and facilitate strand annealing are important for its mechanical function. RAD52 expression varies across tissues but it is prominently observed in rapidly dividing cells and tissues with high rates of DNA repair activity.
Biological function summary

DNA repair protein RAD52 homolog engages significantly in maintaining genome stability. RAD52 forms complexes with other DNA repair proteins such as RAD51 and RPA to perform its role efficiently. The protein supports the loading of RAD51 onto single-stranded DNA a critical step in homologous recombination. By stabilizing RAD51 nucleoprotein filaments RAD52 promotes efficient repair and recombination processes necessary for cellular survival after DNA damage.

Pathways

The activities of RAD52 link to the homologous recombination and DNA damage response pathways. RAD52 operates alongside proteins like RAD51 and BRCA2 to mediate the repair of DNA double-strand breaks through homologous recombination. This relationship is important for maintaining genomic integrity especially during the S and G2 phases of the cell cycle. The synergy between RAD52 and other double-strand break repair proteins ensures effective resolution of DNA damage.

The malfunction or dysregulation of RAD52 connects to conditions like cancer and Fanconi anemia. Deficiencies or mutations in RAD52 can compromise DNA repair and lead to genomic instability a hallmark of cancer. Additionally in Fanconi anemia the interplay between RAD52 and the FA complex is impaired influencing the DNA repair capacity. The disrupted interaction with other repair proteins including those in the BRCA family further suggests RAD52's important role in disease pathogenesis.
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제품 프로토콜

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타겟 정보

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
See full target information RAD52

대체 명칭 보기

DNA repair protein RAD52 homolog, RAD52
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

The Journal of biological chemistry 276:35201-8 PubMed11454867

2001

Homologous pairing promoted by the human Rad52 protein.

Applications

Unspecified application

Species

Unspecified reactive species

W Kagawa,H Kurumizaka,S Ikawa,S Yokoyama,T Shibata
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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