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AB269094

Recombinant human Rad6 protein (Active) (His tag N-Terminus)

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Recombinant human Rad6 protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 152 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

RAD6A, UBE2A, Ubiquitin-conjugating enzyme E2 A, E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, HR6A, hHR6A

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Functional Studies - Recombinant human Rad6 protein (Active) (AB269094)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Rad6 protein (Active) (AB269094)

The specific activity of ab269094 was 30 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human Rad6 protein (Active) (AB269094)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Rad6 protein (Active) (AB269094)

SDS-PAGE analysis of ab269094.

주요 정보

Purity

>95% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab269094 was 30 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

  • 0
  • 0
Primary Antibodies

AB231802

Anti-ACTG2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

  • 5
  • 2
Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Proteins & Peptides

AB135009

Recombinant human MEKK2 protein (GST tag N-Terminus)

Functional Studies - Recombinant human MEKK2 protein (GST tag N-Terminus) (AB135009)

  • 0
  • 0
Proteins & Peptides

AB134555

Recombinant Human alpha smooth muscle Actin protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human alpha smooth muscle Actin protein (His tag N-Terminus) (AB134555)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":152,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P49459","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Rad6 also known as ubiquitin-conjugating enzyme E2 A (UBE2A) is an enzyme with a molecular weight of approximately 17 kDa. This enzyme plays an essential role in the ubiquitination process where it catalyzes the transfer of ubiquitin to substrate proteins. Rad6 is expressed in various tissues with significant activity observed in the testis and brain suggesting its involvement in cell division and development.
Biological function summary

Rad6 functions as part of a larger protein complex involved in the DNA damage repair process. It collaborates with the RAD6 epistasis group which ensures the maintenance of genomic stability. Rad6 as a ubiquitin-conjugating enzyme interacts with E3 ubiquitin ligases facilitating the ubiquitination of target proteins that require degradation or regulation in response to DNA damage. This activity is vital for DNA repair mechanisms like post-replication repair (PRR) which protects cells from mutagenic events.

Pathways

Rad6 integrates into critical biological processes including the ubiquitin-proteasome pathway and the DNA repair pathway. Within the ubiquitin-proteasome system Rad6 aids in tagging proteins for degradation thereby regulating protein levels and cellular functions. It connects to other proteins like E3 enzymes and RAD18 in the DNA repair pathway. Its activity in DNA repair ensures the appropriate response to DNA damage coordinating with other mechanistic proteins to maintain genomic stability.

Rad6 shows significant association with cancer and neurodegenerative conditions. Changes in Rad6 activity can lead to abnormal cell cycle progression contributing to oncogenesis particularly in breast and ovarian cancers. Rad6 interacts with proteins such as BRCA1 a tumor suppressor implicating its role in hereditary breast cancer. Additionally its dysfunction in the brain may relate to Alzheimer's disease highlighting its connection to neuroprotective pathways and protein degradation systems.
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일반 정보

기능

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase (PubMed : 16337599, PubMed : 20061386, PubMed : 23685073, PubMed : 25582440, PubMed : 38297121). In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination (PubMed : 20061386). Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1 (PubMed : 16337599). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation (PubMed : 16337599). Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN (PubMed : 23685073). In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation (PubMed : 38182926). In association with the E3 ubiquitin-protein ligase complex SIFI, inhibits the mitochondrial stress response by acting as a E2 ubiquitin-conjugating enzyme for UBR4 and KCMF1 (PubMed : 38297121).

서열 유사성

Belongs to the ubiquitin-conjugating enzyme family.

Post-translational modifications

Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.

Subcellular localisation

Late endosome

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제품 프로토콜

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타겟 정보

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase (PubMed : 16337599, PubMed : 20061386, PubMed : 23685073, PubMed : 25582440, PubMed : 38297121). In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination (PubMed : 20061386). Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1 (PubMed : 16337599). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation (PubMed : 16337599). Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN (PubMed : 23685073). In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation (PubMed : 38182926). In association with the E3 ubiquitin-protein ligase complex SIFI, inhibits the mitochondrial stress response by acting as a E2 ubiquitin-conjugating enzyme for UBR4 and KCMF1 (PubMed : 38297121).
See full target information UBE2A
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Product promise

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