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AB323073

Recombinant Human RALBP1 Protein Standard (His tag)

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Recombinant Human RALBP1 Protein Standard (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human RALBP1 Protein Standard (His tag) (AB323073)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human RALBP1 Protein Standard (His tag) (AB323073)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human RALBP1 Protein Standard (His tag) (AB323073)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RALBP1 Protein Standard (His tag) (AB323073)

SDS-PAGE analysis of ab323073 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q15311-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Assay Kits

AB309182

Human RALBP1 ELISA Kit

SimpleStep

Sandwich ELISA - Human RALBP1 ELISA Kit (AB309182)

  • 0
  • 0
Primary Antibodies

AB133549

Anti-RALBP1 antibody [EPR6472]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RALBP1 antibody [EPR6472] (AB133549)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"78.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15311","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Multifunctional protein that functions as a downstream effector of RALA and RALB (PubMed : 7673236). As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity (PubMed : 7673236). As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis (PubMed : 10910768, PubMed : 12775724). During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle (PubMed : 12775724). During mitosis, also controls mitochondrial fission as an effector of RALA (PubMed : 21822277). Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L (PubMed : 21822277).. Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.

Post-translational modifications

Tyrosine-phosphorylated upon stimulation of cells with EGF.. May undergo proteolytic cleavage to give peptides which reassemble to form a transporter complex.

Subcellular localisation

Cytoskeleton

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제품 프로토콜

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타겟 정보

Multifunctional protein that functions as a downstream effector of RALA and RALB (PubMed : 7673236). As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity (PubMed : 7673236). As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis (PubMed : 10910768, PubMed : 12775724). During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle (PubMed : 12775724). During mitosis, also controls mitochondrial fission as an effector of RALA (PubMed : 21822277). Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L (PubMed : 21822277).. Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.
See full target information RALBP1

대체 명칭 보기

RLIP, RLIP1, RLIP76, RALBP1, RalA-binding protein 1, RalBP1, 76 kDa Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, Ral-interacting protein 1, DNP-SG ATPase
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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