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AB268947

Recombinant human Rffl protein (Active) (GST tag N-Terminus)

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Recombinant human Rffl protein (Active) (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 363 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

RNF189, RNF34L, RFFL, E3 ubiquitin-protein ligase rififylin, Caspase regulator CARP2, Caspases-8 and -10-associated RING finger protein 2, FYVE-RING finger protein Sakura, RING finger and FYVE-like domain-containing protein 1, RING finger protein 189, RING finger protein 34-like, RING-type E3 ubiquitin transferase rififylin, CARP-2, Fring

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Functional Studies - Recombinant human Rffl protein (Active) (AB268947)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Rffl protein (Active) (AB268947)

The specific activity of ab268947 was 18 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human Rffl protein (Active) (AB268947)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Rffl protein (Active) (AB268947)

SDS-PAGE analysis of ab268947.

주요 정보

Purity

>70% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268947 was 18 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325996

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands) (AB325996)

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Proteins & Peptides

AB95927

Recombinant human NEDD4 protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human NEDD4 protein (DDDDK tag N-Terminus) (AB95927)

  • 0
  • 0
Proteins & Peptides

AB95930

Recombinant human SMURF 2 protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) (AB95930)

  • 0
  • 0
Proteins & Peptides

AB268946

Recombinant human RNF34 protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human RNF34 protein (Active) (AB268946)

  • 0
  • 0
Primary Antibodies

AB209538

Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MWATCCNWFCLDGQPEEVPPPQGARMQAYSNPGYSSFPSPTGLEPSCKSCGAHFANTARKQTCLDCKKNFCMTCSSQVGNGPRLCLLCQRFRATAFQREELMKMKVKDLRDYLSLHDISTEMCREKEELVLLVLGQQPVISQEDRTRASTLSPDFPEQQAFLTQPHSSMVPPTSPNLPSSSAQATSVPPAQVQENQQANGHVSQDQEEPVYLESVARVPAEDETQSIDSEDSFVPGRRASLSDLTDLEDIEGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLVSGAEDQNGGAVPSGLEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMNECPICRQYVIRAVHVFRS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":363,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8WZ73","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Rffl protein also known as RIPLET or RNF135 functions as an E3 ubiquitin ligase. It has a molecular mass of about 53 kDa. Rffl is expressed in many tissues with notable levels in cardiac and skeletal muscle as well as in the brain. The protein contributes to the ubiquitin-proteasome pathway by tagging specific substrates with ubiquitin. This tagging marks them for degradation which regulates protein levels and prevents harmful protein accumulations within cells.
Biological function summary

The Rffl protein participates in the regulation of innate immune responses. It facilitates the polyubiquitination of signaling molecules involved in antiviral pathways. Rffl is not part of a large complex itself but works closely with other proteins to enhance the activation of the immune system's antiviral responses. Its activity modulates the RIG-I-like receptor (RLR) signaling pathway important for the detection of viral RNA by interacting with essential signaling molecules like RIG-I and MAVS.

Pathways

Rffl acts within the signaling cascade related to the RLR pathway and the NF-kappaB pathway. These are essential in mounting an effective immune response against viral infections. Rffl influences the pathway by modulating the function of proteins like TRAF3 and TBK1. Through these interactions it affects the downstream production of type I interferons and other immune response mediators.

The Rffl protein has been linked to immune-related conditions and viral infections. Dysregulation of Rffl activity can lead to impaired antiviral responses which contributes to increased susceptibility to viral diseases. Furthermore studies suggest connections between Rffl and proteins such as IRF3 in the progression or susceptibility to autoimmune diseases. Maintaining proper control of Rffl activity is therefore important for immune system balance and health.
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일반 정보

기능

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.

Post-translational modifications

Autoubiquitinated.. Palmitoylated.. Undergoes caspase-mediated cleavage upon death-receptor activation, by TNFSF10 for instance. May be mediated by the caspases CASP8 and CASP10 in a negative feedback loop.

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제품 프로토콜

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타겟 정보

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
See full target information RFFL
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Product promise

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