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AB268946

Recombinant human RNF34 protein (Active) (GST tag N-Terminus)

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Recombinant human RNF34 protein (Active) (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 372 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human RNF34 protein (Active) (GST tag N-Terminus) (AB268946)
  • FuncS

Supplier Data

Functional Studies - Recombinant human RNF34 protein (Active) (GST tag N-Terminus) (AB268946)

The specific activity of ab268946 was 21 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human RNF34 protein (Active) (GST tag N-Terminus) (AB268946)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human RNF34 protein (Active) (GST tag N-Terminus) (AB268946)

SDS-PAGE analysis of ab268946.

주요 정보

Purity

>80% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268946 was 21 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

Accession

Q969K3-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
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Proteins & Peptides

AB95927

Recombinant human NEDD4 protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human NEDD4 protein (DDDDK tag N-Terminus) (AB95927)

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  • 0
Proteins & Peptides

AB268947

Recombinant human Rffl protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human Rffl protein (Active) (GST tag N-Terminus) (AB268947)

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Proteins & Peptides

AB95930

Recombinant human SMURF 2 protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) (AB95930)

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Proteins & Peptides

AB56278

Recombinant Human p21 protein (GST tag N-Terminus)

Sandwich ELISA - Recombinant Human p21 protein (GST tag N-Terminus) (AB56278)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MKAGATSMWASCCGLLNEVMGTGAVRGQQSAFAGATGPFRFTPNPEFSTYPPAATEGPNIVCKACGLSFSVFRKKHVCCDCKKDFCSVCSVLQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLILRNIPIDTCREKEDLVDLVLCHHGLGSEDDMDTSSLNSSRSQTSSFFTRSFFSNYTAPSATMSSFQGELMDGDQTSRSGVPAQVQSEITSANTEDDDDDDDEDDDDEEENAEDRNPGLSKERVRASLSDLSSLDDVEGMSVRQLKEILARNFVNYSGCCEKWELVEKVNRLYKENEENQKSYGERLQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFKS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":372,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q969K3","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed : 15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed : 17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed : 18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed : 22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed : 25012219).

Post-translational modifications

Autoubiquitinated (in vitro).. Proteolytically cleaved by caspases upon induction of apoptosis by TNF.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed : 15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed : 17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed : 18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed : 22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed : 25012219).
See full target information E3 ubiquitin-protein ligase RNF34

대체 명칭 보기

E3 ubiquitin-protein ligase RNF34, Caspase regulator CARP1, Caspases-8 and -10-associated RING finger protein 1, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RING finger protein 34, RING finger protein RIFF, RING-type E3 ubiquitin transferase RNF34, CARP-1, hRFI, RNF34
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Product promise

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