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AB140736

Recombinant Human RPS10 protein (His tag N-Terminus)

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Recombinant Human RPS10 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

Small ribosomal subunit protein eS10, 40S ribosomal protein S10, RPS10

1 이미지
SDS-PAGE - Recombinant Human RPS10 protein (His tag N-Terminus) (AB140736)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RPS10 protein (His tag N-Terminus) (AB140736)

15% SDS-PAGE analysis of ab140736 (3 µg).

주요 정보

Purity

>85% SDS-PAGE

ab140736 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB151550

Anti-RPS10 antibody [EPR8545]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as Ribosomal protein S10
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ","proteinLength":"Full Length","predictedMolecularWeight":"21 kDa","actualMolecularWeight":null,"aminoAcidEnd":165,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46783","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPS10 also known as 40S ribosomal protein S10 is a component of the small ribosomal subunit essential for protein synthesis. It weighs approximately 18.7 kDa. The protein is expressed in various tissues including the liver kidney and brain. Its primary role involves participating in the assembly and function of ribosomes by interacting with rRNA and supporting the translation of mRNA into amino acids.
Biological function summary

RPS10 contributes to the formation and stability of the ribosomal 40S subunit. It works as part of the ribosome complex important for initiating and propelling protein synthesis. This ribosomal complex performs a central role in cellular growth and proliferation as it aids in assembling polypeptide chains that proteins need for diverse cellular processes. By doing so it supports a wide range of physiological functions.

Pathways

RPS10 contributes to protein synthesis through the translational initiation and elongation pathways. Its participation supports the accurate decoding and synthesis of proteins which are critical to cellular metabolism and growth. RPS10 interacts with other ribosomal proteins such as RPS12 and RPS14 to maintain these pathways' function and efficiency helping regulate cellular homeostasis and response to environmental stimuli.

RPS10 mutations can link to Diamond-Blackfan Anemia a condition marked by failed red blood cell production impacting patient health. Moreover aberrations in RPS10 link to various cancers such as breast cancer due to its role in protein synthesis and cell growth. RPS10 interacts with proteins like RPL5 another ribosomal protein within these disease contexts suggesting a broader contribution of ribosomal dysfunction in disease pathogenesis.
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일반 정보

기능

Component of the 40S ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399).

서열 유사성

Belongs to the eukaryotic ribosomal protein eS10 family.

Post-translational modifications

Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.. Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, PubMed:28132843, PubMed:28685749, PubMed:32011234, PubMed:36302773). Deubiquitinated by OTUD3 and USP21, antagonizing ZNF598 activity (PubMed:32011234). Deubiquitinated by OTUD1, antagonizing ZNF598 activity and stimulating formation of polysomes: deubiquitination by OTUD1 promotes stability and translation of a subset mRNAs with a high abundance of rare codons can limit the translation rate (PubMed:36445135). Deubiquitinated by USP10 (PubMed:31981475).

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Component of the 40S ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399).
See full target information 40S ribosomal protein S10
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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