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AB99326

Recombinant Human SAHH protein (His tag N-Terminus)

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(2 제품이 사용된 논문 )

Recombinant Human SAHH protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 432 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, WB.
2 이미지
Western blot - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)
  • WB

Unknown

Western blot - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)

All lanes:

Anti-SAHH antibody (<a href='/ko/products/unavailable/sahh-antibody-ab56146'>ab56146</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human SAHH protein (His tag N-Terminus) (ab99326) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 90s

SDS-PAGE - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)

15% SDS-PAGE showing ab99326 (3μg).

주요 정보

Purity

>95% SDS-PAGE

ab99326 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE, WB

applications

Biologically active

No

Accession

P23526-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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  • 0
Proteins & Peptides

AB79190

Recombinant Human GNMT protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GNMT protein (His tag N-Terminus) (AB79190)

  • 0
  • 0
Proteins & Peptides

AB285970

Recombinant Human CBS protein (Active)

Functional Studies - Recombinant Human CBS protein (Active) (AB285970)

  • 0
  • 0
Proteins & Peptides

AB139234

Recombinant Human MST protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human MST protein (His tag N-Terminus) (AB139234)

  • 0
  • 0
Proteins & Peptides

AB89356

Recombinant Human TST protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TST protein (His tag N-Terminus) (AB89356)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab99326 can be used as a WB positive control in conjunction with <a href='/ko/products/unavailable/sahh-antibody-ab56146'>ab56146</a>.</p>" } } }
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제품 세부 정보

This product was previously labelled as S adenosylhomocysteine hydrolase
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY","proteinLength":"Full Length","predictedMolecularWeight":"49.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":432,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23526","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

S-Adenosylhomocysteine hydrolase also known as SAHH plays a critical mechanical role in cellular metabolism. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to homocysteine and adenosine. This enzyme approximately 48 kDa in mass is ubiquitously expressed in most tissues highlighting its universal importance in biological systems. SAHH maintains the cellular methylation balance by regulating the concentrations of SAH a byproduct of methylation reactions.
Biological function summary

S-Adenosylhomocysteine hydrolase acts as an important player in the methylation processes within the cell. By breaking down SAH it ensures proper methylation of DNA proteins and lipids through the recycling of homocysteine and adenosine. SAHH operates as a homotetramer forming a complex essential for its enzymatic function. The balance maintained by SAHH in cellular methylation allows for proper gene expression and signal transduction within various cellular processes.

Pathways

S-Adenosylhomocysteine hydrolase plays an essential role in the methionine cycle and one-carbon metabolism pathways. These pathways are interconnected with the folate cycle important for nucleotide synthesis and cellular methylation. SAHH works with enzymes like methionine synthase and adenosylmethionine linking it to regulation of homocysteine levels. These interconnections are fundamental for maintaining cellular function and homeostasis.

S-Adenosylhomocysteine hydrolase dysregulation can have significant implications. Hyperhomocysteinemia a condition characterized by high levels of homocysteine in the blood often results from SAHH dysfunction leading to cardiovascular diseases. Additionally aberrant SAHH activity connects to certain types of cancer as altered methylation patterns can disrupt normal cell proliferation. The interplay of SAHH with other proteins such as methylenetetrahydrofolate reductase (MTHFR) illustrates the complex network within which this enzyme operates affecting disease outcomes.
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일반 정보

기능

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).

서열 유사성

Belongs to the adenosylhomocysteinase family.

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제품 프로토콜

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타겟 정보

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).
See full target information AHCY

대체 명칭 보기

SAHH, AHCY, Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Assay and drug development technologies 12:258-71 PubMed24927133

2014

A sensitive luminescent assay for the histone methyltransferase NSD1 and other SAM-dependent enzymes.

Applications

FuncS

Species

Unspecified reactive species

Katherine M Drake,Venita G Watson,Anne Kisielewski,Rebecca Glynn,Andrew D Napper

Analytical chemistry 84:3593-8 PubMed22416759

2012

Luciferase-based assay for adenosine: application to S-adenosyl-L-homocysteine hydrolase.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuel S Burgos,Shivali A Gulab,María B Cassera,Vern L Schramm
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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