Recombinant Human Scramblase 1 protein (His tag N-Terminus)
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Recombinant Human Scramblase 1 protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
대체 명칭 보기
Phospholipid scramblase 1, PL scramblase 1, Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, Mg(2+)-dependent nuclease, MmTRA1b, PLSCR1
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human Scramblase 1 protein (His tag N-Terminus) (AB136366)
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Scramblase 1 protein (His tag N-Terminus) (AB136366)
15% SDS-PAGE analysis of ab136366 (3 μg)
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Reactivity 정보
서열 정보
특성 및 보관 정보
제형
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분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Scramblase 1 mediates phospholipid scrambling in response to increased intracellular calcium levels facilitating changes in cell membrane morphology. It performs this function independently and as part of specific protein complexes that regulate membrane curvature and vesicle fusion. The role of PLSCR1 extends beyond membrane activities; it has been linked to signal transduction and the immune response suggesting a broader impact on cellular dynamics.
Pathways
Scramblase 1 influences critical biological processes like blood coagulation and apoptosis. It interacts with proteins such as annexin V which stabilizes membrane phospholipids and regulates cell death pathways. In the coagulation cascade PLSCR1 has been shown to interact with factors involved in platelet activation demonstrating its regulatory role in maintaining hemostasis and tissue homeostasis.
일반 정보
기능
Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer leaflet of the plasma membrane resulting in collapse of the phospholipid asymmetry which leads to phosphatidylserine externalization on the cell surface (PubMed : 10770950, PubMed : 18629440, PubMed : 23590222, PubMed : 23659204, PubMed : 24343571, PubMed : 24648509, PubMed : 29748552, PubMed : 32110987, PubMed : 8663431, PubMed : 9218461, PubMed : 9485382, PubMed : 9572851). Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with TRPC5 (By similarity). Also exhibits magnesium-dependent nuclease activity against double-stranded DNA and RNA but not single-stranded DNA and can enhance DNA decatenation mediated by TOP2A (PubMed : 17567603, PubMed : 27206388). Negatively regulates FcR-mediated phagocytosis in differentiated macrophages (PubMed : 26745724). May contribute to cytokine-regulated cell proliferation and differentiation (By similarity). May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes (PubMed : 15308695). Inhibits the functions of viral transactivators, including human T-cell leukemia virus (HTLV)-1 protein Tax, human immunodeficiency virus (HIV)-1 Tat, human hepatitis B virus (HBV) HBx, Epstein-Barr virus (EBV) BZLF1 and human cytomegalovirus IE1 and IE2 proteins through direct interactions (PubMed : 22789739, PubMed : 23501106, PubMed : 25365352, PubMed : 31434743, PubMed : 35138119). Also mediates the inhibition of influenza virus infection by preventing nuclear import of the viral nucleoprotein/NP (PubMed : 29352288, PubMed : 35595813). Plays a crucial role as a defense factor against SARS-CoV-2 independently of its scramblase activity by directly targeting nascent viral vesicles to prevent virus-membrane fusion and the release of viral RNA into the host-cell cytosol (PubMed : 37438530).. (Microbial infection) Acts as an attachment receptor for HCV.
서열 유사성
Belongs to the phospholipid scramblase family.
Post-translational modifications
Phosphorylation at Thr-161 by PKC/PKCD increases its phospholipid scramblase activity during both cell stimulation and apoptosis (PubMed:10770950). Phosphorylated by OXSR1 in the presence of RELT.. Palmitoylation is required for its phospholipid scramblase activity (PubMed:9572851). Palmitoylation regulates its localization to the cell membrane or the nucleus; trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus (PubMed:12564925).
Subcellular localisation
Nucleus
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