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AB87664

Recombinant Human Securin protein (His tag N-Terminus)

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(2 제품이 사용된 논문 )

Recombinant Human Securin protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >80%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Human Securin protein (His tag N-Terminus) (AB87664)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Securin protein (His tag N-Terminus) (AB87664)

ab87664 on 15% SDS-PAGE (3μg)

주요 정보

Purity

>80% SDS-PAGE

Purified by using conventional chromatography techniques.

Endotoxin 레벨

< 1 EU/µg

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O95997-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.029% EDTA, 0.0017% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

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Primary Antibodies

AB79546

Anti-Securin antibody [EPR3240]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Securin antibody [EPR3240] (AB79546)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95997","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Securin also known as PTTG1 (pituitary tumor-transforming gene 1) is a protein involved in the regulation of sister chromatid separation during cell division. It inhibits Separase an enzyme important for this separation. Securin has a molecular mass of approximately 22 kDa. Its expression is notable in various tissues including the pituitary gland and cancerous tissues which suggests a role in tumorigenesis.
Biological function summary

Securing proper chromosome segregation Securin forms a complex with Separase. By inhibiting Separase Securin ensures that separation of chromatids occurs only at the correct stage of mitosis. This regulatory function is critical for maintaining genomic stability and avoiding aneuploidy which can lead to tumorigenesis. The presence of Securin in dividing cells highlights its role in the cell cycle.

Pathways

Securin functions are integrated within the cell cycle and apoptosis pathways. In the cell cycle pathway Securin controls the metaphase-anaphase transition. This involves interaction with cyclin-dependent kinases (CDKs) and the anaphase-promoting complex (APC/C). In terms of apoptosis Securin may interact with proteins like Bcl-2 impacting cell survival and death decisions under stress conditions.

Securin has been associated with cancer particularly pituitary adenomas and colorectal cancer. Its overexpression can lead to chromosomal instability a hallmark of many cancers. Furthermore interactions with other proteins like p53 known for its tumor-suppressing activities suggest a complex interplay in oncogenic processes where disruptions can contribute to tumor development and progression.
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일반 정보

기능

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

서열 유사성

Belongs to the securin family.

Post-translational modifications

Phosphorylated at Ser-165 by CDK1 during mitosis.. Phosphorylated in vitro by ds-DNA kinase.. Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
See full target information PTTG1

대체 명칭 보기

EAP1, PTTG, TUTR1, PTTG1, Securin, Esp1-associated protein, Pituitary tumor-transforming gene 1 protein, Tumor-transforming protein 1, hPTTG
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

ACS chemical biology 17:2605-2618 PubMed35952650

2022

Proximity Labeling Reveals Spatial Regulation of the Anaphase-Promoting Complex/Cyclosome by a Microtubule Adaptor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofu Cao,Adnan Shami Shah,Ethan J Sanford,Marcus B Smolka,Jeremy M Baskin

Nature communications 13:2089 PubMed35440621

2022

Regulated interaction of ID2 with the anaphase-promoting complex links progression through mitosis with reactivation of cell-type-specific transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Bae Lee,Luciano Garofano,Aram Ko,Fulvio D'Angelo,Brulinda Frangaj,Danika Sommer,Qiwen Gan,KyeongJin Kim,Timothy Cardozo,Antonio Iavarone,Anna Lasorella
제품이 사용된 논문 모두 보기
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Product promise

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