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AB125586

Recombinant Human SIRT3 protein (GST tag N-Terminus)

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Recombinant Human SIRT3 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 399 aa range, expressed in Escherichia coli, with >75%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Human SIRT3 protein (GST tag N-Terminus) (AB125586)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SIRT3 protein (GST tag N-Terminus) (AB125586)

SDS Page analysis of ab125586 running at approximately 66 kDa.

주요 정보

Purity

>75% Densitometry

Purity was determined to be >75% by densitometry.

발현 시스템

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NTG7-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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  • 0
Proteins & Peptides

AB167964

Recombinant human CREBBP protein (GST tag N-Terminus)

Functional Studies - Recombinant human CREBBP protein (GST tag N-Terminus) (AB167964)

  • 0
  • 0
Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
Primary Antibodies

AB133534

Anti-Rad51 antibody [EPR4030(3)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534)

  • 4
  • 15
Primary Antibodies

AB76420

Anti-RPA32/RPA2 antibody [EPR2877Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":399,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NTG7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SIRT3 also known as Sirtuin 3 and mitochondrial sirtuin is a deacetylase with a mass of roughly 44 kDa. This protein is mostly found in the mitochondria where it regulates the acetylation state of various substrates. It plays an important role in maintaining mitochondrial homeostasis by modifying protein activity. SIRT3 is evolutionarily conserved and highly expressed in tissues with high energy demand like the heart liver and brown adipose tissue.
Biological function summary

SIRT3 modulates energy homeostasis by deacetylating and regulating enzymes involved in metabolism. It is a part of the mitochondrial sirtuin family contributing to fatty acid oxidation and the antioxidant defense system. By targeting key metabolic enzymes SIRT3 directly impacts the tricarboxylic acid (TCA) cycle and oxidative phosphorylation processes. These functions highlight its role in maintaining energy efficiency and reducing reactive oxidative species.

Pathways

SIRT3 participates in vital metabolic pathways such as the TCA cycle and the urea cycle. It interacts with other proteins like MnSOD and IDH2 to enhance mitochondrial function and overall cellular metabolism. This helps control the balance between energy production and consumption integrating signals from the AMPK and NAD+-dependent pathways to coordinate responses to changes in energy availability.

SIRT3 has implications in metabolic syndrome and cancer. Reduced SIRT3 activity is associated with increased susceptibility to metabolic disturbances like obesity and type 2 diabetes. Its deficiency affects the regulation of ROS and metabolic adaptation linking it to tumorigenesis and cancer progression. As it connects with proteins like HIF-1α in hypoxic conditions SIRT3 plays a role in cellular adaptation to stress influencing disease progression.
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일반 정보

기능

NAD-dependent protein deacetylase (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 19535340, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090, PubMed : 38146092). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, MRPL12 and the ATP synthase subunit ATP5PO (PubMed : 16788062, PubMed : 18680753, PubMed : 19535340, PubMed : 24121500, PubMed : 24252090, PubMed : 38146092). Contributes to the regulation of the cellular energy metabolism (PubMed : 24252090). Important for regulating tissue-specific ATP levels (PubMed : 18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed : 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis (By similarity). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (By similarity). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups, such as benzoyl and lactoyl, leading to protein debenzoylation and delactylation, respectively (PubMed : 39524354, PubMed : 36896611, PubMed : 37720100). Catalyzes debenzoylation of PPIF and ACLY (PubMed : 37720100). Mediates delactylation of CCNE2 and 'Lys-16' of histone H4 (H4K16la) (PubMed : 36896611, PubMed : 37720100).

서열 유사성

Belongs to the sirtuin family. Class I subfamily.

Post-translational modifications

Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.

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제품 프로토콜

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타겟 정보

NAD-dependent protein deacetylase (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 19535340, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090, PubMed : 38146092). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, MRPL12 and the ATP synthase subunit ATP5PO (PubMed : 16788062, PubMed : 18680753, PubMed : 19535340, PubMed : 24121500, PubMed : 24252090, PubMed : 38146092). Contributes to the regulation of the cellular energy metabolism (PubMed : 24252090). Important for regulating tissue-specific ATP levels (PubMed : 18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed : 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis (By similarity). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (By similarity). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups, such as benzoyl and lactoyl, leading to protein debenzoylation and delactylation, respectively (PubMed : 39524354, PubMed : 36896611, PubMed : 37720100). Catalyzes debenzoylation of PPIF and ACLY (PubMed : 37720100). Mediates delactylation of CCNE2 and 'Lys-16' of histone H4 (H4K16la) (PubMed : 36896611, PubMed : 37720100).
See full target information SIRT3

대체 명칭 보기

SIR2L3, SIRT3, hSIRT3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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