AB63176

Recombinant Human Smad2 protein (GST tag N-Terminus)

Be the first to review this product! 리뷰 제출

(0 출판물)

Recombinant Human Smad2 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

2 이미지

Unknown

Western blot - Recombinant Human Smad2 protein (GST tag N-Terminus) (AB63176)

All lanes:

Western blot - Anti-Smad2 antibody [7A5] (<a href='/ko/products/primary-antibodies/smad2-antibody-7a5-ab71109'>ab71109</a>) at 1/2000 dilution

All lanes:

Western blot - Recombinant Human Smad2 protein (GST tag N-Terminus) (ab63176) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 52 kDa

true

Exposure time: 10s

SDS-PAGE - Recombinant Human Smad2 protein (GST tag N-Terminus) (AB63176)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Smad2 protein (GST tag N-Terminus) (AB63176)

ab63176 on SDS-PAGE, MW ~90kDa.

주요 정보

Purity

>90% Densitometry

발현 시스템

Escherichia coli

Applications

SDS-PAGE, WB

applications

Biologically active

Accession

Q15796-1

Animal free

Carrier free

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab63176 can be used as a WB positive control in conjunction with <a href='/ko/products/primary-antibodies/smad2-antibody-7a5-ab71109'>ab71109</a>.</p>" } } }

style

left-column

style

left-column

서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15796","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

style

left-column

특성 및 보관 정보

제형

Liquid

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

False

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 also known as Mothers against decapentaplegic homolog 2 (MAD2) or MADR2 is a signaling protein involved in the transforming growth factor-beta (TGF-β) receptor pathway. Smad2 has a molecular mass of approximately 58 kDa and expresses in various tissues including epithelial mesenchymal and endothelial cells. Smad2 undergoes phosphorylation on serine residues in response to TGF-β signaling converting it into phosphorylated forms often referred to as p-Smad2 or phospho-Smad2. These phosphorylated forms are critical for the relay of signals from the cell surface to the nucleus.

Biological function summary

Smad2 acts as an intracellular mediator for TGF-β signaling a pathway important for regulating cell proliferation differentiation and apoptosis. Smad2 typically functions as part of a heteromeric complex with Smad4 another key player in TGF-β signaling. Upon activation phosphorylated Smad2 combines with Smad4 to form a complex that translocates into the nucleus. This complex then binds to specific DNA sequences to regulate the transcription of target genes involved in processes such as cell growth inhibition and extracellular matrix production.

Pathways

Smad2 is integral to the TGF-β and activin receptor signaling pathways. These pathways are essential in controlling cell growth and immune responses. Smad2 interacts with other proteins such as Smad3 in addition to Smad4 to modulate gene expression effectively. The interaction between Smad2 and these proteins ensures precise control of cellular responses to external stimuli emphasizing its pivotal role in maintaining cellular homeostasis.

Smad2 correlates with various pathological conditions including fibrosis and cancer. Aberrant Smad2 signaling can contribute to the development of these diseases as excessive TGF-β signaling promotes fibrotic tissue deposition and tumor progression. Smad2 connects with other proteins like Smad3 in these pathological contexts both acting as mediators of abnormal cellular behaviors. Understanding the regulatory mechanisms of Smad2 can help develop therapeutic strategies against disorders linked to dysregulated TGF-β signaling.

style

left-column

일반 정보

기능

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).

서열 유사성

Belongs to the dwarfin/SMAD family.

Post-translational modifications

Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1.. In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation. Monoubiquitinated, leading to prevent DNA-binding (By similarity). Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes (PubMed:21947082). Ubiquitinated by RNF111, leading to its degradation: only SMAD2 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD2 and regulating its turnover (By similarity).. Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.

style

left-column

제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

See full target information SMAD2

대체 명칭 보기

MADH2, MADR2, SMAD2, SMAD family member 2, SMAD 2, hSMAD2, JV18-1, Mad-related protein 2, Mothers against decapentaplegic homolog 2, hMAD-2, MAD homolog 2, Mothers against DPP homolog 2

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant Human Smad2 protein (GST tag N-Terminus)

Western blot - Recombinant Human Smad2 protein (GST tag N-Terminus) (AB63176)

SDS-PAGE - Recombinant Human Smad2 protein (GST tag N-Terminus) (AB63176)

주요 정보

Purity

발현 시스템

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Proteins & Peptides

Proteins & Peptides

Primary Antibodies

Proteins & Peptides

Reactivity 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

일반 정보

기능

서열 유사성

Post-translational modifications

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise