Recombinant Human SREBP2 protein (GST tag N-Terminus)

일반 정보

기능

Sterol regulatory element-binding protein 2. Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed : 32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed : 32322062).. Processed sterol regulatory element-binding protein 2. Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis (PubMed : 12177166, PubMed : 32322062). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed : 12177166, PubMed : 7903453). Regulates transcription of genes related to cholesterol synthesis pathway (PubMed : 12177166, PubMed : 32322062).

서열 유사성

Belongs to the SREBP family.

Post-translational modifications

Sterol regulatory element-binding protein 2. Processed in the Golgi apparatus, releasing the protein from the membrane (PubMed:10805775, PubMed:32322062, PubMed:8626610, PubMed:9651382). At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum (PubMed:10805775, PubMed:32322062, PubMed:8626610, PubMed:9651382). In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease (PubMed:10805775, PubMed:32322062, PubMed:8626610, PubMed:9651382). The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane (PubMed:10805775, PubMed:9651382). Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Cleavage and activation is induced by mediated cholesterol efflux (PubMed:8643593).. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.. Processed sterol regulatory element-binding protein 2. Ubiquitinated; the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus.

Subcellular localisation

Nucleus