Recombinant Human ST2 Protein Standard (His tag)
Be the first to review this product! 리뷰 제출
|
(0 출판물)
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Human ST2 Protein Standard (His tag) (AB316652)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human ST2 Protein Standard (His tag) (AB316652)
SDS-PAGE analysis of ab316652 under reducing conditions for 2ug protein.
보완 제품
Reagents, Controls & Accessories
AB116027
Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)
- 5
- 2
Reactivity 정보
제품 세부 정보
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
서열 정보
특성 및 보관 정보
제형
Purification 테크닉
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.
Pathways
IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.
일반 정보
기능
Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
서열 유사성
Belongs to the interleukin-1 receptor family.
Post-translational modifications
Ubiquitinated at Lys-321 in a FBXL19-mediated manner; leading to proteasomal degradation. Ubiquitination by TRAF6 via 'Lys-27'-linked polyubiquitination and deubiquitination by USP38 serves as a critical regulatory mechanism for fine-tuning IL1RL1-mediated inflammatory response (PubMed:35238669).
타겟 정보
대체 명칭 보기
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com