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AB43618

Recombinant Human STAT3 protein (GST tag N-Terminus)

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(10 제품이 사용된 논문 )

Recombinant Human STAT3 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for WB.
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Western blot - Recombinant Human STAT3 protein (GST tag N-Terminus) (AB43618)
  • WB

Unknown

Western blot - Recombinant Human STAT3 protein (GST tag N-Terminus) (AB43618)

Demonstration of protein purity (>90%) by western blot.

All lanes:

Western blot - Recombinant Human STAT3 protein (GST tag N-Terminus) (ab43618)

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주요 정보

Purity

>70%

Purified by affinity chromatography

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB

applications

Biologically active

No

Accession

P40763-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.00174% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

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Proteins & Peptides

AB43616

Recombinant Human STAT1 protein (GST tag N-Terminus)

  • 0
  • 0
Proteins & Peptides

AB42619

Recombinant human JAK2 protein (His tag N-Terminus)

Functional Studies - Recombinant human JAK2 protein (His tag N-Terminus) (AB42619)

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  • 0
Proteins & Peptides

AB182829

Recombinant Human STAT5b protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human STAT5b protein (GST tag N-Terminus) (AB182829)

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  • 0
Proteins & Peptides

AB84634

Recombinant Human STAT4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human STAT4 protein (His tag N-Terminus) (AB84634)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Recombinant full-length human STAT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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서열 정보

[{"linker":null,"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFMAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM","proteinLength":"Full Length","predictedMolecularWeight":"120 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P40763","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.
Biological function summary

STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.

Pathways

STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.

Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.
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제품 프로토콜

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타겟 정보

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed : 10688651, PubMed : 12359225, PubMed : 12873986, PubMed : 15194700, PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18242580, PubMed : 18782771, PubMed : 22306293, PubMed : 23084476, PubMed : 28262505, PubMed : 32929201, PubMed : 38404237). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18782771, PubMed : 28262505, PubMed : 32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed : 12359225). Activated by IL31 through IL31RA (PubMed : 15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg) : acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed : 28065600, PubMed : 28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed : 17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed : 18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed : 23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).
See full target information STAT3

대체 명칭 보기

APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor
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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록and refine your search

Redox biology 71:103124 PubMed38503216

2024

Endogenous sulfur dioxide deficiency as a driver of cardiomyocyte senescence through abolishing sulphenylation of STAT3 at cysteine 259.

Applications

Unspecified application

Species

Unspecified reactive species

Shangyue Zhang,Bingquan Qiu,Boyang Lv,Guosheng Yang,Yinghong Tao,Yongyan Hu,Kun Li,Xiaoqi Yu,Chaoshu Tang,Junbao Du,Hongfang Jin,Yaqian Huang

Nucleic acids research 51:1050-1066 PubMed36660824

2023

A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xianli Du,Jing Pang,Bin Gu,Tian Si,Yan Chang,Tianqi Li,Min Wu,Zicheng Wang,Yuxia Wang,Jiannan Feng,Ning Wu,Jianghong Man,Huiyan Li,Ailing Li,Tong Zhang,Bo Wang,Xiaotao Duan

Phytotherapy research : PTR 37:809-819 PubMed36447385

2022

Saikosaponin D alleviates cancer cachexia by directly inhibiting STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Lin Chen,Liu-Yuan Xia,Jun-Ping Zhang,Yan Wang,Jian-Yu Chen,Cheng Guo,Wei-Heng Xu

Frontiers in pharmacology 13:836724 PubMed35712699

2022

Novel STAT3 Inhibitors Targeting STAT3 Dimerization by Binding to the STAT3 SH2 Domain.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Hua,Xing Yuan,Yun-Heng Shen,Jinxin Wang,Waqas Azeem,Shuo Yang,Alexandra Gade,Seyed Mohammad Lellahi,Anne Margrete Øyan,Xisong Ke,Wei-Dong Zhang,Karl-Henning Kalland

Frontiers in oncology 11:664403 PubMed34055630

2021

Carnosol Is a Novel Inhibitor of p300 Acetyltransferase in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Halima Alsamri,Hussain El Hasasna,Bincy Baby,Aysha Alneyadi,Yusra Al Dhaheri,Mohammed Akli Ayoub,Ali H Eid,Ranjit Vijayan,Rabah Iratni

Molecules (Basel, Switzerland) 25: PubMed32422984

2020

Trichothecin Inhibits Cancer-Related Features in Colorectal Cancer Development by Targeting STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Qi,Meng Li,Xiao-Min Zhang,Xiu-Fen Dai,Jian Cui,De-Hai Li,Qian-Qun Gu,Zhi-Hua Lv,Jing Li

Pharmacological research 158:104871 PubMed32413482

2020

Imperatorin alleviates cancer cachexia and prevents muscle wasting via directly inhibiting STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Chen,Weiheng Xu,Quanjun Yang,Hong Zhang,Lili Wan,Bo Xin,Junping Zhang,Cheng Guo

Cell host & microbe 27:41-53.e6 PubMed31862381

2019

Salmonella Effector SteE Converts the Mammalian Serine/Threonine Kinase GSK3 into a Tyrosine Kinase to Direct Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ioanna Panagi,Elliott Jennings,Jingkun Zeng,Regina A Günster,Cullum D Stones,Hazel Mak,Enkai Jin,Daphne A C Stapels,Nur Z Subari,Trung H M Pham,Susan M Brewer,Samantha Y Q Ong,Denise M Monack,Sophie Helaine,Teresa L M Thurston

Redox biology 23:101175 PubMed31129031

2019

15-Keto prostaglandin E suppresses STAT3 signaling and inhibits breast cancer cell growth and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Ji Lee,Su-Jung Kim,Young-Il Hahn,Hyo-Jin Yoon,Bitnara Han,Kyeojin Kim,Seungbeom Lee,Kwang Pyo Kim,Young Ger Suh,Hye-Kyung Na,Young-Joon Surh

The Journal of biological chemistry 288:15971-9 PubMed23576436

2013

Reciprocal regulation of protein kinase and pyruvate kinase activities of pyruvate kinase M2 by growth signals.

Applications

Unspecified application

Species

Unspecified reactive species

Xueliang Gao,Haizhen Wang,Jenny J Yang,Jing Chen,Jiang Jie,Liangwei Li,Yinwei Zhang,Zhi-Ren Liu
제품이 사용된 논문 모두 보기
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