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AB140417

Recombinant Human Sumo 1 protein (His tag N-Terminus)

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Recombinant Human Sumo 1 protein (His tag N-Terminus) is a Human Full Length protein, in the 2 to 97 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.
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Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab109196, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (<a href='/ko/products/primary-antibodies/sumo-2-sumo-3-sumo-4-antibody-epr3002-ab109196'>ab109196</a>) at 1/200 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/ko/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 16 kDa

true

Exposure time: 20s

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab126606, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (<a href='/ko/products/primary-antibodies/sumo-2-sumo-3-sumo-4-antibody-epr7163-ab126606'>ab126606</a>) at 1/5000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/ko/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 16 kDa

true

Exposure time: 3s

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab109005, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (<a href='/ko/products/primary-antibodies/sumo-2-sumo-3-antibody-epr4602-ab109005'>ab109005</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/ko/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 16 kDa

true

Exposure time: 20s

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab133352, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 1 antibody [EP298] (<a href='/ko/products/primary-antibodies/sumo-1-antibody-ep298-ab133352'>ab133352</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (His tag N-Terminus) (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/ko/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/ko/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 16 kDa

true

Exposure time: 10s

SDS-PAGE - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Sumo 1 protein (His tag N-Terminus) (AB140417)

SDS-PAGE analysis of ab140417.

주요 정보

Purity

>90% Densitometry

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P63165-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Proteins & Peptides

AB268952

Recombinant human SAE1 + UBA2 protein (Active) (His tag N-Terminus, GST tag N-Terminus)

Functional Studies - Recombinant human SAE1 + UBA2 protein (Active) (AB268952)

  • 0
  • 0
Proteins & Peptides

AB269091

Recombinant human UBE1 protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human UBE1 protein (Active) (AB269091)

  • 0
  • 0
Proteins & Peptides

AB269103

Recombinant human UBE2I / UBC9 protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)

  • 0
  • 0
Proteins & Peptides

AB290069

Recombinant Human PP1A Protein

Mass Spectrometry - Recombinant Human PP1A Protein (AB290069)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"SDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGG","proteinLength":"Full Length","predictedMolecularWeight":"19 kDa","actualMolecularWeight":null,"aminoAcidEnd":97,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P63165","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SUMO 1 also known as small ubiquitin-like modifier 1 is part of the protein family involved in post-translational modification. The protein mechanically adds a SUMO group to target proteins through sumoylation a process similar to ubiquitination. SUMO 1 typically has a molecular mass of around 11 kDa. It expresses in various tissues and cells including the nucleus and cytoplasm of eukaryotic cells. In addition it is important in processes like nuclear transport transcriptional regulation and protein stabilization.
Biological function summary

SUMO 1 contributes significantly to maintaining cellular homeostasis. It is an integral part of a SUMOylation complex that modifies other proteins to alter their function localization or interactions. Through its modification actions SUMO 1 affects processes such as DNA repair and the cell cycle. By interacting with components of the nuclear pore complex and transcription factors SUMO 1 modulates essential biological activities at multiple levels within the cell.

Pathways

This protein plays a significant role in the PI3K/Akt pathway and Wnt signaling. SUMO 1 associates with proteins like RanGAP1 and various transcription factors highlighting the complexity of its regulatory actions. These associations are important for the regulation of cell survival proliferation and differentiation. SUMOylation by SUMO 1 has been linked to an interference with phosphorylation showing its potential influence on cell signaling pathways.

The dysregulation of SUMO 1 is seen in cancer and neurodegenerative diseases. For instance altered SUMOylation patterns contribute to the progression of certain cancers. Furthermore SUMO 1 interacts with proteins like p53 and these interactions can affect tumor suppression and cell cycle regulation. In neurodegenerative disorders disturbed SUMOylation is associated with protein aggregation and neuronal damage implicating SUMO 1 in diseases like Alzheimer's.
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일반 정보

기능

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed : 19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed : 24651376).

서열 유사성

Belongs to the ubiquitin family. SUMO subfamily.

Post-translational modifications

Cleavage of precursor form by SENP1 or SENP2 is necessary for function.. Polymeric SUMO1 chains undergo polyubiquitination by RNF4.

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제품 프로토콜

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타겟 정보

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed : 19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed : 24651376).
See full target information SUMO1

대체 명칭 보기

SMT3C, SMT3H3, UBL1, OK/SW-cl.43, SUMO1, Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, GMP1, Smt3C
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Product promise

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