Recombinant Human TAF1 protein (GST tag N-Terminus)
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Recombinant Human TAF1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 1519 to 1651 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
대체 명칭 보기
BA2R, CCG1, CCGS, TAF2A, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, p250, TAF(II)250, TAFII-250, TAFII250
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human TAF1 protein (GST tag N-Terminus) (AB198119)
4-20% SDS-PAGE stained with Coomassie Blue.
Lane 1 : ab198119 (2.7 μg)
Lane 2 : Protein Marker
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추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TAF1 interacts extensively with other TFIID components to control the formation of the pre-initiation complex on core promoters. This protein is integral to the regulation of gene expression by modulating transcriptional activity in cells. TAF1 acts as a histone acetyltransferase and a kinase modifying other proteins' functions and structural capabilities in the transcription process. Its enzymatic activities make it essential for the phosphorylation and acetylation of histones directly impacting the accessibility of chromatin for transcription.
Pathways
TAF1 functions prominently in the RNA polymerase II transcription initiation pathway. Its interactions with TATA-binding protein and other TAFs facilitate the robust expression of genes essential for various cellular functions. TAF1 establishes connections with proteins like TBP acting within the canonical pathway that guides precise transcription initiation. Additionally TAF1 partakes in the p53 signaling pathway where it interacts with several p53 regulators influencing cell cycle regulation and apoptosis.
일반 정보
기능
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly (PubMed : 25412659, PubMed : 27007846, PubMed : 33795473). TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (PubMed : 33795473). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (PubMed : 25412659, PubMed : 8625415). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (PubMed : 25412659). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (PubMed : 25412659). Possesses DNA-binding activity (PubMed : 25412659). Essential for progression of the G1 phase of the cell cycle (PubMed : 11278496, PubMed : 15053879, PubMed : 2038334, PubMed : 8450888, PubMed : 8625415, PubMed : 9660973, PubMed : 9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed : 15870300).
서열 유사성
Belongs to the TAF1 family.
Post-translational modifications
Phosphorylated by casein kinase II in vitro.
타겟 정보
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