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AB256153

Recombinant human Tau (mutated P301S) protein monomer (Active) (Tag Free)

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Recombinant human Tau (mutated P301S) protein monomer (Active) (Tag Free) is a Human Full Length protein, in the 1 to 441 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, FuncS.
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Functional Studies - Recombinant human Tau (mutated P301S) protein monomer (Active) (Tag Free) (AB256153)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Tau (mutated P301S) protein monomer (Active) (Tag Free) (AB256153)

Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (ab256153). A greater increase in fluorescence is seen when 50μM monomer (ab256153) is combined with 10 nM PFFs (ab246003 or ab256152), as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm.

SDS-PAGE - Recombinant human Tau (mutated P301S) protein monomer (Active) (Tag Free) (AB256153)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Tau (mutated P301S) protein monomer (Active) (Tag Free) (AB256153)

SDS-PAGE - Recombinant human Tau (mutated P301 S) protein (Active) (ab256153).

주요 정보

Purity

>95%

발현 시스템

Escherichia coli

Tags

Tag free

Applications

WB, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (ab256153). A greater increase in fluorescence is seen when 50μM monomer (ab256153) is combined with 10 nM PFFs (ab246003 or ab256152), as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm.

Accession

P10636-8

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: 0.58% Sodium chloride, 0.24% HEPES

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

  • 0
  • 0
Proteins & Peptides

AB269022

Recombinant Human Tau (phospho ) protein (Tag Free)

SDS-PAGE - Recombinant Human Tau (phospho ) protein (AB269022)

  • 0
  • 0
Proteins & Peptides

AB254309

Recombinant human Alpha-Synuclein protein filament

Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament (AB254309)

  • 0
  • 0
Primary Antibodies

AB281588

Anti-MAP2 antibody [RM1010] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

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  • 0
Proteins & Peptides

AB114149

Recombinant Human GFAP protein (GST tag N-Terminus)

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Monomer.
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서열 정보

[{"linker":null,"sequence":"MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVSGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL","proteinLength":"Full Length","predictedMolecularWeight":"45.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":441,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10636","tags":[]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Ion exchange chromatography
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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제품 프로토콜

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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT mutated P301S

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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