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AB156345

Recombinant Human TDP43 protein (denatured) (His tag N-Terminus)

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(3 제품이 사용된 논문 )

Recombinant Human TDP43 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 414 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

TDP43, TARDBP, TAR DNA-binding protein 43, TDP-43

1 이미지
SDS-PAGE - Recombinant Human TDP43 protein (denatured) (His tag N-Terminus) (AB156345)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TDP43 protein (denatured) (His tag N-Terminus) (AB156345)

15% SDS-PAGE analysis of 3µg ab156345.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Primary Antibodies

AB109535

Anti-TDP43 antibody [EPR5810]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-TDP43 antibody [EPR5810] (AB109535)

  • 5
  • 1
Proteins & Peptides

AB316609

Recombinant Human Neurogranin Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Neurogranin Protein Standard (His tag) (AB316609)

  • 0
  • 0
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Proteins & Peptides

AB316441

Recombinant Human Tau Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Tau Protein Standard (His tag) (AB316441)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM","proteinLength":"Full Length","predictedMolecularWeight":"48.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":414,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13148","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.
Biological function summary

TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.

Pathways

Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.

The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.
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일반 정보

기능

RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed : 23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed : 23519609, PubMed : 24240615, PubMed : 24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed : 21358640, PubMed : 29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed : 28794432). Also regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed : 30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed : 19765185, PubMed : 23398327). Also participates in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed : 30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed : 27123980). Negatively regulates the expression of CDK6 (PubMed : 19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed : 25678563).

Post-translational modifications

Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Phosphorylated upon cellular stress.. Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.. Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

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제품 프로토콜

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타겟 정보

RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed : 23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed : 23519609, PubMed : 24240615, PubMed : 24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed : 21358640, PubMed : 29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed : 28794432). Also regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed : 30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed : 19765185, PubMed : 23398327). Also participates in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed : 30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed : 27123980). Negatively regulates the expression of CDK6 (PubMed : 19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed : 25678563).
See full target information TARDBP
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta neuropathologica communications 9:15 PubMed33461623

2021

Spreading of TDP-43 pathology via pyramidal tract induces ALS-like phenotypes in TDP-43 transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebing Ding,Zhi Xiang,Chi Qin,Yongkang Chen,Haiyan Tian,Lin Meng,Danhao Xia,Han Liu,Jia Song,Jun Fu,Mingming Ma,Xuejing Wang

Cellular and molecular life sciences : CMLS 78:1745-1763 PubMed32780150

2020

ITSN1 regulates SAM68 solubility through SH3 domain interactions with SAM68 proline-rich motifs.

Applications

Unspecified application

Species

Unspecified reactive species

S Pankivskyi,D Pastré,E Steiner,V Joshi,A Rynditch,L Hamon

Frontiers in cellular neuroscience 14:117 PubMed32477070

2020

Cytoplasmic TDP43 Binds microRNAs: New Disease Targets in Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ximena Paez-Colasante,Claudia Figueroa-Romero,Amy E Rumora,Junguk Hur,Faye E Mendelson,John M Hayes,Carey Backus,Ghislaine F Taubman,Laurie Heinicke,Nils G Walter,Sami J Barmada,Stacey A Sakowski,Eva L Feldman
제품이 사용된 논문 모두 보기
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Product promise

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