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AB283419

Recombinant human TGF alpha protein (Active)

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Recombinant human TGF alpha protein (Active) is a Human Full Length protein, in the 24 to 98 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, FuncS, HPLC, Cell Culture.

대체 명칭 보기

Protransforming growth factor alpha, TGFA

4 이미지
Biochemical assay - Recombinant human TGF alpha protein (Active) (AB283419)
  • Biochemical assay

Supplier Data

Biochemical assay - Recombinant human TGF alpha protein (Active) (AB283419)

Fully biologically active determined by the dose dependent proliferation of Balb/c-3T3 cells. ED50 <= 0.20 ng/ml, corresponding to a specific activity of 5x106 units/mg.

Mass Spectrometry - Recombinant human TGF alpha protein (Active) (AB283419)
  • Mass Spec

Unknown

Mass Spectrometry - Recombinant human TGF alpha protein (Active) (AB283419)

Mass determination by ESI-TOF.

Predicted MW is 8057 (+/- 10Da by ESI-TOF). MW observed is 8058.65.

SDS-PAGE - Recombinant human TGF alpha protein (Active) (AB283419)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TGF alpha protein (Active) (AB283419)

SDS-PAGE analysis of ab283419

HPLC - Recombinant human TGF alpha protein (Active) (AB283419)
  • HPLC

Supplier Data

HPLC - Recombinant human TGF alpha protein (Active) (AB283419)

HPLC analysis of ab283419

주요 정보

Purity

>95% HPLC

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

HPLC, FuncS, Cell Culture, Mass Spec, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent proliferationof Balb/c-3T3 cells. ED50 is ≤ 0.20 ng/ml, corresponding to a specific activity of 5 x 106 units/mg.

Accession

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

서열 정보

[{"linker":null,"sequence":"ENSTSPLSADPPVAAAVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLAVVAASQKKQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":98,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01135","tags":[]}]

특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True

일반 정보

기능

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

제품 프로토콜

타겟 정보

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
See full target information Protransforming growth factor alpha

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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