AB51064

Recombinant human Thioredoxin / TRX protein (Active) (Tag Free)

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(6 제품이 사용된 논문 )

Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) is a Human Full Length protein, in the 1 to 105 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, WB.

2 이미지

Unknown

Western blot - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)

All lanes:

Western blot - Anti-Thioredoxin / TRX antibody (<a href='/ko/products/primary-antibodies/thioredoxin-trx-antibody-ab26320'>ab26320</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (ab51064) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 12 kDa

true

Exposure time: 30s

SDS-PAGE - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)

3 ug of reduced ab51064, on 15% SDS-PAGE, stained with Coomassie Blue.

주요 정보

Purity

>95% SDS-PAGE

Recombinant human Thioredoxin / TRX was overexpressed in E. coli and purified by using conventional chromatography techniques.

발현 시스템

Escherichia coli

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Accession

P10599-1

Animal free

Carrier free

Species

Human

보관 버퍼

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab51064 can be used as a WB positive control in conjunction with <a href='/ko/products/primary-antibodies/thioredoxin-trx-antibody-ab26320'>ab26320</a>." } } }

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제품 세부 정보

MW confirmed by MALDI-TOF.

Concentration determined by BCA assay.

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서열 정보

[{"linker":null,"sequence":"MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV","proteinLength":"Full Length","predictedMolecularWeight":"11.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":105,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10599","tags":[]}]

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특성 및 보관 정보

제형

Liquid

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

True

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin (TRX) also known as TXN or Trx is a small redox protein with a molecular mass of approximately 12 kDa. It acts as an oxidoreductase enzyme facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxin is ubiquitously expressed across various tissues indicating its function in a broad range of cellular activities. The protein comprises an active site with a conserved Cys-Gly-Pro-Cys sequence critical for its reducing activity.

Biological function summary

Thioredoxin influences cellular redox homeostasis and plays direct roles in regulating cell growth and apoptosis. It participates as a major reductant in cells influencing transcription factors and enzymatic activities that depend on thiol-disulfide exchanges. Thioredoxin can interact with other components like thioredoxin reductase and NADPH to form the thioredoxin system a powerful antioxidant defense mechanism. Diseases typically arise from abnormal regulation of this system highlighting its influence on cellular survival and proliferation.

Pathways

Thioredoxin integrates into significant signaling and metabolic processes. It is an important player in the cellular response to oxidative stress and participates in signaling pathways such as the MAPK and apoptosis pathways. In these pathways thioredoxin reduces the oxidative stress transcription factor AP-1 influencing cell fate decisions. Its interaction with thioredoxin-interacting protein (TXNIP) further modulates cellular responses to oxidative environments.

Altered thioredoxin activity associates with cancer and neurodegenerative diseases. Its overexpression links to tumor progression where it promotes survival and resistance to apoptosis. Additionally in the context of neurodegenerative disorders thioredoxin’s interaction with amyloid-beta peptides implicates it in Alzheimer’s disease by mitigating oxidative stress damage. Through these conditions its role as a protector against oxidative stress remains pivotal.

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일반 정보

기능

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed : 17182577, PubMed : 19032234, PubMed : 2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed : 16408020, PubMed : 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed : 11118054, PubMed : 9108029).. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

서열 유사성

Belongs to the thioredoxin family.

Post-translational modifications

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

Subcellular localisation

Nucleus

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information TXN

대체 명칭 보기

TRDX, TRX, TRX1, TXN, Thioredoxin, Trx, ATL-derived factor, Surface-associated sulphydryl protein, ADF, SASP

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Clinical cancer research : an official journal of 25:7162-7174 PubMed31527169

2019

Bioactivation of Napabucasin Triggers Reactive Oxygen Species-Mediated Cancer Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Fieke E M Froeling,Manojit Mosur Swamynathan,Astrid Deschênes,Iok In Christine Chio,Erin Brosnan,Melissa A Yao,Priya Alagesan,Matthew Lucito,Juying Li,An-Yun Chang,Lloyd C Trotman,Pascal Belleau,Youngkyu Park,Harry A Rogoff,James D Watson,David A Tuveson

Nature communications 10:4073 PubMed31501427

2019

Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emre Balta,Robert Hardt,Jie Liang,Henning Kirchgessner,Christian Orlik,Beate Jahraus,Stefan Hillmer,Stefan Meuer,Katrin Hübner,Guido H Wabnitz,Yvonne Samstag

Journal of applied physiology (Bethesda, Md. : 198 127:858-866 PubMed31246554

2019

Characterization of extracellular redox enzyme concentrations in response to exercise in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Alex J Wadley,Gary Keane,Tom Cullen,Lynsey James,Jordan Vautrinot,Matthew Davies,Bethan Hussey,David J Hunter,Sarabjit Mastana,Adrian Holliday,Steen V Petersen,Nicolette C Bishop,Martin R Lindley,Steven J Coles

Cell chemical biology 26:449-461.e8 PubMed30713096

2019

Selective Disruption of Mitochondrial Thiol Redox State in Cells and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Lee M Booty,Justyna M Gawel,Filip Cvetko,Stuart T Caldwell,Andrew R Hall,John F Mulvey,Andrew M James,Elizabeth C Hinchy,Tracy A Prime,Sabine Arndt,Cristiane Beninca,Thomas P Bright,Menna R Clatworthy,John R Ferdinand,Hiran A Prag,Angela Logan,Julien Prudent,Thomas Krieg,Richard C Hartley,Michael P Murphy

Chemistry Central journal 7:150 PubMed24007191

2013

Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Barreto Duarte Carilho Torrao,Irundika Hk Dias,Stuart J Bennett,Christopher R Dunston,Helen R Griffiths

PloS one 6:e21926 PubMed21779355

2011

Orally active multi-functional antioxidants are neuroprotective in a rat model of light-induced retinal damage.

Applications

Species

Unspecified reactive species

James Randazzo,Zifeng Zhang,Michael Hoff,Hiroyoshi Kawada,Andrew Sachs,Yang Yuan,Neena Haider,Peter Kador

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant human Thioredoxin / TRX protein (Active) (Tag Free)

Western blot - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)

SDS-PAGE - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)

주요 정보

Purity

발현 시스템

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Secondary Antibodies

Reactivity 정보

제품 세부 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

일반 정보

기능

서열 유사성

Post-translational modifications

Subcellular localisation

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise