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AB271761

Recombinant human TIGIT protein (Active) (Biotin) (Fc tag C-Terminus + Avi tag C-Terminus)

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Recombinant human TIGIT protein (Active) (Biotin) (Fc tag C-Terminus + Avi tag C-Terminus) is a Human Fragment protein, in the 22 to 141 aa range, expressed in HEK 293 cells, with >90%, suitable for FuncS, SDS-PAGE.

대체 명칭 보기

VSIG9, VSTM3, TIGIT, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

2 이미지
Functional Studies - Recombinant human TIGIT protein (Active) (Biotin) (Fc tag C-Terminus + Avi tag C-Terminus) (AB271761)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TIGIT protein (Active) (Biotin) (Fc tag C-Terminus + Avi tag C-Terminus) (AB271761)

Specific activity of ab271761.

SDS-PAGE - Recombinant human TIGIT protein (Active) (Biotin) (Fc tag C-Terminus + Avi tag C-Terminus) (AB271761)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TIGIT protein (Active) (Biotin) (Fc tag C-Terminus + Avi tag C-Terminus) (AB271761)

SDS-PAGE analysis of 3 μg ab271761.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

Fc tag C-Terminus Avi tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Assay conditions: 100 nM of ab271761 was incubated for 1 hour with the indicated concentration of CD155 in a 10 μl reaction in with TIGIT Assay Buffer. Chelate Acceptor beads were added and incubated for 30 mins followed by Strep-donor beads for 30 mins and alpha counts were read.

Conjugation

Biotin

Excitation/Emission
Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

storage-buffer

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보완 제품

Proteins & Peptides

AB283906

Recombinant Human CTLA4 protein

SDS-PAGE - Recombinant Human CTLA4 protein (AB283906)

  • 0
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AB205800

Recombinant human BTK protein (His tag N-Terminus)

Functional Studies - Recombinant human BTK protein (AB205800)

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Proteins & Peptides

AB235737

Recombinant Human Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 protein (Tagged)

SDS-PAGE - Recombinant Human Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 protein (Tagged) (AB235737)

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Primary Antibodies

AB243903

Anti-TIGIT antibody [BLR047F] - BSA free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [BLR047F] - BSA free (AB243903)

  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This protein runs at a higher MW by SDS-PAGE due to glycosylation.</p>" } } }
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제품 세부 정보

Enzymatically biotin-labeled using Avi-tag™ technology
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서열 정보

[{"linker":null,"sequence":"MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP","proteinLength":"Fragment","predictedMolecularWeight":"41.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":141,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q495A1","tags":[{"tag":"Fc","terminus":"C-Terminus"},{"tag":"Avi","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle|Store in the dark
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.
Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.
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일반 정보

기능

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed : 19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed : 23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed : 24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed : 32503945).

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제품 프로토콜

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타겟 정보

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed : 19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed : 23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed : 24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed : 32503945).
See full target information TIGIT
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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