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AB287940

Recombinant Human TIMP2 protein

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Recombinant Human TIMP2 protein is a Human Full Length protein, in the 27 to 220 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC.

대체 명칭 보기

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

3 이미지
Mass Spectrometry - Recombinant Human TIMP2 protein (AB287940)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human TIMP2 protein (AB287940)

ESI-TOF analysis ab287940.

Predicted MW is 21812.05 Da (+/- 1-0 Da by ESI TOF). Observed MW is 21812.41 Da.

SDS-PAGE - Recombinant Human TIMP2 protein (AB287940)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TIMP2 protein (AB287940)

SDS-PAGE analysis of ab287940

HPLC - Recombinant Human TIMP2 protein (AB287940)
  • HPLC

Supplier Data

HPLC - Recombinant Human TIMP2 protein (AB287940)

HPLC analysis of ab287940.

주요 정보

Purity

>95% SDS-PAGE

Purity by HPLC >=95%

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

HPLC, Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

Yes

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

  • 5
  • 3
Proteins & Peptides

AB280945

Recombinant human TIMP1 protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human TIMP1 protein (Active) (AB280945)

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  • 0
Proteins & Peptides

AB285962

Recombinant Human MMP3 protein

  • 0
  • 0
Assay Kits

AB270213

Human TIMP2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP2 ELISA Kit (AB270213)

  • 5
  • 1
Proteins & Peptides

AB196093

Recombinant Mouse Collagen III protein (His tag)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP","proteinLength":"Full Length","predictedMolecularWeight":"21.81 kDa","actualMolecularWeight":"21.81 kDa","aminoAcidEnd":220,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16035","tags":[]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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일반 정보

기능

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

서열 유사성

Belongs to the protease inhibitor I35 (TIMP) family.

Post-translational modifications

The activity of TIMP2 is dependent on the presence of disulfide bonds.

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제품 프로토콜

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타겟 정보

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
See full target information TIMP2
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Product promise

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