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AB235820

Recombinant Human TPOR/MPL protein (His tag)

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Recombinant Human TPOR/MPL protein (His tag) is a Human Fragment protein, in the 26 to 491 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

CD110, TPOR, MPL, Thrombopoietin receptor, TPO-R, Myeloproliferative leukemia protein, Proto-oncogene c-Mpl

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SDS-PAGE - Recombinant Human TPOR/MPL protein (His tag) (AB235820)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TPOR/MPL protein (His tag) (AB235820)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab235820.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

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Secondary Antibodies

AB191866

VHH Single Domain Anti-Rabbit IgG Fc (HRP)

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (AB191866)

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  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as TPOR
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서열 정보

[{"linker":null,"sequence":"QDVSLLASDSEPLKCFSRTFEDLTCFWDEEEAAPSGTYQLLYAYPREKPRACPLSSQSMPHFGTRYVCQFPDQEEVRLFFPLHLWVKNVFLNQTRTQRVLFVDSVGLPAPPSIIKAMGGSQPGELQISWEEPAPEISDFLRYELRYGPRDPKNSTGPTVIQLIATETCCPALQRPHSASALDQSPCAQPTMPWQDGPKQTSPSREASALTAEGGSCLISGLQPGNSYWLQLRSEPDGISLGGSWGSWSLPVTVDLPGDAVALGLQCFTLDLKNVTCQWQQQDHASSQGFFYHSRARCCPRDRYPIWENCEEEEKTNPGLQTPQFSRCHFKSRNDSIIHILVEVTTAPGTVHSYLGSPFWIHQAVRLPTPNLHWREISSGHLELEWQHPSSWAAQETCYQLRYTGEGHQDWKVLEPPLGARGGTLELRPRSRYRLQLRARLNGPTYQGPWSSWSDPTRVETATETAW","proteinLength":"Fragment","predictedMolecularWeight":"56.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":491,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P40238","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The target TPOR/MPL also known as the MPL protein or Myeloproliferative Leukemia protein functions as a receptor for thrombopoietin (TPO). It is composed of 635 amino acids with a molecular weight of approximately 70 kDa. This protein is expressed primarily on the surface of hematopoietic progenitor cells megakaryocytes and platelets. The receptor a cell surface-bound protein plays a significant role by binding to TPO facilitating cellular signaling involved in cell proliferation and maturation.
Biological function summary

The TPOR/MPL protein promotes the growth and differentiation of multiple blood cell types through direct interactions with TPO. When TPO binds to MPL it triggers a signaling cascade essential for the regulation of platelet production and maintenance of hematopoiesis. This receptor forms part of a larger receptor complex that includes JAK2 protein kinase which further transduces signals for cell growth and survival. The engagement of JAK2 enhances the functional capability of MPL leading to the activation of various downstream effectors.

Pathways

The TPOR/MPL protein is central to the thrombopoietin signaling pathway facilitating the activation of downstream molecules like STAT3 and STAT5. These transcription factors activate gene expression critical for cell proliferation and survival. MPL is closely related to other hematopoietic receptors within the Jak-STAT pathway. The pathway is integral for hematopoietic stem cell function and involves interactions with proteins like EPO receptor which utilizes similar pathways for erythropoiesis.

The TPOR/MPL protein is implicated in myeloproliferative disorders such as essential thrombocythemia and primary myelofibrosis. Mutations in the MPL gene can alter receptor function leading to uncontrolled cell proliferation. Such mutations often present in tandem with mutations in other proteins like JAK2 amplifying the disease processes. The inappropriate activation of the MPL signaling pathway results in excessive production of platelets or fibrosis within the bone marrow contributing significantly to disease phenotypes.
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일반 정보

기능

Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K (PubMed : 15899890, PubMed : 37633268). In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production.

서열 유사성

Belongs to the type I cytokine receptor family. Type 1 subfamily.

Post-translational modifications

Phosphorylated at Tyr-591 in response to THPO stimulation.. Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination.

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제품 프로토콜

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타겟 정보

Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K (PubMed : 15899890, PubMed : 37633268). In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production.
See full target information MPL
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Product promise

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