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AB236161

Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (Tagged)

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Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (Tagged) is a Human Full Length protein, in the 2 to 471 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

IFI53, WARS, WRS, WARS1, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase, IFP53, TrpRS, hWRS

1 이미지
SDS-PAGE - Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (Tagged) (AB236161)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (Tagged) (AB236161)

ab236161 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

10x His tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Proteins & Peptides

AB133145

Recombinant Human FABP4 protein

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Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB228724

Anti-Tryptophanyl tRNA synthetase/WRS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

  • 4
  • 1
Proteins & Peptides

AB132315

Recombinant Human PRAME protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human PRAME protein (GST tag N-Terminus) (AB132315)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Previously labelled as Tryptophanyl tRNA synthetase.
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서열 정보

[{"linker":null,"sequence":"PNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":471,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23381","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophanyl tRNA synthetase also known as WRS or TrpRS is an enzyme responsible for attaching tryptophan to its corresponding tRNA during protein synthesis. This enzyme is approximately 53 kDa in mass. It ensures the accurate translation of the genetic code in mRNA into proteins by catalyzing the esterification of tryptophan to the tRNA^Trp. WRS is mostly found in the cytoplasm of cells across various tissues contributing to its role in protein biosynthesis.
Biological function summary

WRS is involved in the important process of protein translation ensuring fidelity in the genetic code transfer from RNA to protein. As part of the aminoacyl-tRNA synthetases family WRS helps in maintaining the overall function and stability of the translational apparatus. It is not typically known to be part of any larger protein complex. In addition to its core function in protein synthesis WRS possesses non-canonical roles related to immune response regulation and angiogenesis.

Pathways

WRS plays key roles in the aminoacyl-tRNA biosynthesis and mTOR signaling pathways. These pathways are essential for regulating protein synthesis and cell growth. In the mTOR pathway WRS's function relates to its interaction with proteins involved in cell growth and proliferation. Its roles within these pathways highlight its influence on cellular homeostasis and protein regulation interacting closely with other synthetases and transcription factors.

WRS has associations with certain cancers and autoimmune disorders. Abnormal expression of WRS is observed in various cancer types reflecting its contribution to dysregulated protein synthesis and cell growth. Additionally WRS has been linked to autoimmune diseases like dermatomyositis where its immune-regulatory roles become compromised. In these conditions WRS's interactions with proteins such as interferons illustrate its importance in the pathophysiology of inflammation and immune response.
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일반 정보

기능

Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction : tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).. Isoform 1. Has no angiostatic activity.. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed : 11773625, PubMed : 11773626, PubMed : 14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed : 14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed : 14630953).. Isoform 2. Has an angiostatic activity.

서열 유사성

Belongs to the class-I aminoacyl-tRNA synthetase family.

Post-translational modifications

Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.

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제품 프로토콜

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타겟 정보

Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction : tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).. Isoform 1. Has no angiostatic activity.. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed : 11773625, PubMed : 11773626, PubMed : 14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed : 14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed : 14630953).. Isoform 2. Has an angiostatic activity.
See full target information WARS1
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Product promise

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