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AB159720

Recombinant Human Tspan-8 protein (GST tag N-Terminus)

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Recombinant Human Tspan-8 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 237 aa range, expressed in Wheat germ, suitable for ELISA, WB.

대체 명칭 보기

TM4SF3, TSPAN8, Tetraspanin-8, Tspan-8, Transmembrane 4 superfamily member 3, Tumor-associated antigen CO-029

1 이미지
SDS-PAGE - Recombinant Human Tspan-8 protein (GST tag N-Terminus) (AB159720)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Tspan-8 protein (GST tag N-Terminus) (AB159720)

ab159720 on a 12.5% SDS-PAGE stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

  • 0
  • 0
Primary Antibodies

AB70007

Anti-Tspan-8 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-8 antibody (AB70007)

  • 4
  • 1
Primary Antibodies

AB315816

Anti-P5CS antibody [EPR28827-56]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody [EPR28827-56] (AB315816)

  • 0
  • 0
Secondary Antibodies

AB99697

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP)

VeriBlot

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (AB99697)

  • 5
  • 1
Biochemicals

AB146284

Sudan black B, Fat-soluble diazo dye

Chemical Structure - Sudan black B, Fat-soluble diazo dye (AB146284)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as TM4SF3.
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서열 정보

[{"linker":null,"sequence":"MAGVSACIKYSMFTFNFLFWLCGILILALAIWVRISNDSQAIFGSEDVGSSSYVAVDILIAVGAIIMILGFLACCGAIKESRCMLLLFFIGLLLILLLQVATGILGAVFKSKSDRIVNETLYENTKLLSATGESEKQFQEAIIVFQEEFKCCGLVNGAADWGNNFQHYPELCACLDKQRPCQSYNGKQVYKETCISFIKDFLAKNLIIVIGIAFGLAVIEILGLVFSMVLYCQIGNK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":237,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P19075","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tspan-8 also known as Tetraspanin-8 is a protein with a mass of about 27 kDa. This protein belongs to the tetraspanin family characterized by four transmembrane domains. Tspan-8 is expressed in various tissues including the gastrointestinal tract kidney and lung with notable expression in human tumors. It localizes to membranes where it participates in organizing specific microdomains essential for cellular activities like adhesion and migration.
Biological function summary

Tspan-8 impacts several cellular functions such as motility and invasion. It often acts as part of a complex interacting with integrins and other tetraspanins. Through these interactions Tspan-8 facilitates communication between the extracellular matrix and the cytoskeleton. This interaction affects processes like cellular attachment and extracellular matrix remodeling contributing to its functions in normal physiological and pathological conditions.

Pathways

Numerous studies tie Tspan-8 to significant biological pathways particularly those involved in signal transduction and cellular movement. It connects closely with the integrin signaling pathway influencing cellular responses to external signals. In this context Tspan-8 interacts with proteins such as integrin αvβ6 contributing to diverse cellular outcomes. Additionally its role within the epithelial-to-mesenchymal transition (EMT) pathway is relevant for its function in cell plasticity and migration.

Tspan-8 links to cancer and inflammatory conditions. Its overexpression associates with tumor progression and metastasis particularly in cancers like colorectal and pancreatic cancer. The protein's interactions with integrin partners notably integrin αvβ6 could enhance cancer cell invasiveness. Moreover inflammation-related diseases might involve Tspan-8 through its influence on inflammatory cell trafficking and invasion contributing to disease pathology. Understanding these interactions can provide insights into novel therapeutic targets.
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일반 정보

기능

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 27180357, PubMed : 36078095). Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking (PubMed : 25761241). Promotes ADAM17-mediated TNF processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs) (PubMed : 36078095). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration (PubMed : 25761241). Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR (PubMed : 35904232). Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration (PubMed : 37204469). Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin (PubMed : 37835445).

서열 유사성

Belongs to the tetraspanin (TM4SF) family.

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제품 프로토콜

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타겟 정보

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 27180357, PubMed : 36078095). Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking (PubMed : 25761241). Promotes ADAM17-mediated TNF processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs) (PubMed : 36078095). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration (PubMed : 25761241). Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR (PubMed : 35904232). Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration (PubMed : 37204469). Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin (PubMed : 37835445).
See full target information TSPAN8
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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