JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB269092

Recombinant human Uba6 protein (Active) (GST tag N-Terminus)

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Recombinant human Uba6 protein (Active) (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 1052 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.
2 이미지
Functional Studies - Recombinant human Uba6 protein (Active) (GST tag N-Terminus) (AB269092)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Uba6 protein (Active) (GST tag N-Terminus) (AB269092)

The specific activity of ab269092 was 19 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human Uba6 protein (Active) (GST tag N-Terminus) (AB269092)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Uba6 protein (Active) (GST tag N-Terminus) (AB269092)

SDS-PAGE analysis of ab269092.

주요 정보

Purity

>75% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab269092 was 19 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.

Accession

A0AVT1-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

style
left-column

보완 제품

Primary Antibodies

AB228918

Anti-Uba6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uba6 antibody (AB228918)

  • 0
  • 0
Primary Antibodies

AB209976

Anti-MyoD1 antibody

Lab Essentials

IHC - Wholemount - Anti-MyoD1 antibody (AB209976)

  • 0
  • 0
Primary Antibodies

AB177956

Anti-MMACHC/CblC antibody [EPR11268]

RabMAb|Recombinant

Immunoprecipitation - Anti-MMACHC/CblC antibody [EPR11268] (AB177956)

  • 0
  • 0
Primary Antibodies

AB313933

Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (AB313933)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

서열 정보

[{"linker":null,"sequence":"MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHDTD","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1052,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"A0AVT1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Uba6 also known as ubiquitin-like modifier activating enzyme 6 is an E1-like ubiquitin-activating enzyme. It has an approximate mass of around 120 kDa. This enzyme facilitates the activation of ubiquitin and similar proteins by catalyzing the formation of a ubiquitin-adenylate intermediate. Uba6 is expressed in a wide range of human tissues with higher levels observed in the brain heart and skeletal muscle. Its expression suggests a need for its activity in diverse biological processes across different tissue types.
Biological function summary

Uba6 modifies proteins by attaching ubiquitin moieties which tags them for various fates including degradation or location changes. Uba6 functions as a part of the ubiquitin-proteasome system (UPS) a critical protein degradation and quality control pathway. In this system Uba6 collaborates with E2 conjugating enzymes such as Ube2z which transfer ubiquitin from Uba6 to substrate proteins. This involvement emphasizes its role in protein homeostasis and cellular stress responses by controlling protein stability.

Pathways

Uba6 takes part in the maintenance of protein homeostasis and also influences the NEDD8 modification pathway. The UPS pathway integrates Uba6 with processes involving protein turnover and stress responses maintaining cellular oxidative stress levels. In the NEDD8 pathway Uba6 interacts with proteins like UBE2D which influence protein degradation scenarios that differ from those managed by the classic ubiquitin system. These pathways highlight the diverse regulatory roles Uba6 holds in managing intracellular protein dynamics.

Uba6 plays a role in neurodegenerative diseases and some cancers. In Alzheimer's disease Uba6 affects the accumulation of misfolded proteins connecting its action to the degradation pathways that include related ubiquitination enzymes. It also shows relevance to tumor progression in cancers where aberrations in ubiquitin-mediated pathways play a part in unregulated cell growth. The functions of Uba6 in these conditions link it with disorders of protein homeostasis where proteins like UBE2Z are involved.
style
left-column

일반 정보

기능

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed : 35970836, PubMed : 35986001). Specific for ubiquitin, does not activate ubiquitin-like peptides. Also activates UBD/FAT10 conjugation via adenylation of its C-terminal glycine (PubMed : 17889673, PubMed : 35970836, PubMed : 35986001). Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.

서열 유사성

Belongs to the ubiquitin-activating E1 family.

style
left-column

제품 프로토콜

style
left-column

타겟 정보

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed : 35970836, PubMed : 35986001). Specific for ubiquitin, does not activate ubiquitin-like peptides. Also activates UBD/FAT10 conjugation via adenylation of its C-terminal glycine (PubMed : 17889673, PubMed : 35970836, PubMed : 35986001). Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
See full target information UBA6

대체 명칭 보기

MOP4, UBE1L2, UBA6, Ubiquitin-like modifier-activating enzyme 6, Ubiquitin-activating enzyme 6, Monocyte protein 4, Ubiquitin-activating enzyme E1-like protein 2, MOP-4, E1-L2
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Leukemia 39:1997-2009 PubMed40588566

2025

Characterization of E1 enzyme dependencies in mutant-UBA1 human cells reveals UBA6 as a novel therapeutic target in VEXAS syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Courtnee A Clough,Claire Cunningham,Sophia Y Philbrook,Kathleen M Hueneman,Avery M Sampson,Kwangmin Choi,Kenneth D Greis,Daniel Starczynowski
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com