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AB269093

Recombinant human Ube1L protein (Active) (GST tag N-Terminus)

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Recombinant human Ube1L protein (Active) (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 1012 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human Ube1L protein (Active) (GST tag N-Terminus) (AB269093)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Ube1L protein (Active) (GST tag N-Terminus) (AB269093)

The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.

SDS-PAGE - Recombinant human Ube1L protein (Active) (GST tag N-Terminus) (AB269093)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Ube1L protein (Active) (GST tag N-Terminus) (AB269093)

SDS-PAGE analysis of ab269093.

주요 정보

Purity

>95% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.

Accession

P41226-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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  • 1
Proteins & Peptides

AB269116

Recombinant human USP15 protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human USP15 protein (Active) (GST tag N-Terminus) (AB269116)

  • 0
  • 0
Proteins & Peptides

AB269108

Recombinant human UBE2O protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human UBE2O protein (Active) (GST tag N-Terminus) (AB269108)

  • 0
  • 0
Proteins & Peptides

AB269106

Recombinant human Ube2L6 protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human Ube2L6 protein (Active) (His tag N-Terminus) (AB269106)

  • 0
  • 0
Proteins & Peptides

AB78929

Recombinant Human ISG15 protein (Calmodulin tag N-Terminus)

SDS-PAGE - Recombinant Human ISG15 protein (Calmodulin tag N-Terminus) (AB78929)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1012,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P41226","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ube1L also known as ubiquitin-activating enzyme E1-like plays an important role in the ubiquitin-proteasome system by activating ubiquitin molecules. With a molecular mass of approximately 117 kDa Ube1L functions as the initial enzyme in the ubiquitination cascade catalyzing the formation of a thioester bond with ubiquitin. This process is important for the tagging of proteins for degradation. Ube1L exhibits widespread expression in various tissues indicating its importance in diverse cellular activities.
Biological function summary

This protein participates actively in the regulation of protein homeostasis. Ube1L helps to manage protein turnover and maintains protein quality control by marking damaged or unneeded proteins for degradation via the proteasome. Although Ube1L functions primarily as a standalone enzyme it cooperates closely with ubiquitin-conjugating and -ligating enzymes (E2s and E3s) to achieve precise ubiquitination of target proteins. This cooperation is critical for cellular processes like cell cycle regulation and response to stress.

Pathways

Ube1L is a significant element in the ubiquitin-proteasome pathway which is essential for protein degradation and turnover. It also contributes to the regulation of interferon signaling by modifying proteins such as ISG15 a ubiquitin-like protein involved in antiviral response. Related proteins such as UBE2L6 and UBE2E1 serve as E2 conjugating enzymes that interact with Ube1L further influencing these pathways by facilitating the transfer of activated ubiquitin.

Ube1L has been implicated in certain types of cancers and viral infections. Alterations in the expression or function of Ube1L can lead to disrupted protein degradation contributing to cancer progression through the accumulation of oncogenic proteins. Ube1L is also connected to proteins like ISG15 and USP18 which are involved in host defense mechanisms against viral infections. Dysregulation in these contexts can result in inadequate antiviral responses or improper regulation of cell proliferation.
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일반 정보

기능

E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation (PubMed : 27545325). Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus (PubMed : 16254333, PubMed : 19073728, PubMed : 29056542, PubMed : 29743376, PubMed : 37722521). For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition (PubMed : 20133869). ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling (PubMed : 27564865).

서열 유사성

Belongs to the ubiquitin-activating E1 family.

Post-translational modifications

ISGylated.. Ubiquitinated by RNF170.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation (PubMed : 27545325). Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus (PubMed : 16254333, PubMed : 19073728, PubMed : 29056542, PubMed : 29743376, PubMed : 37722521). For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition (PubMed : 20133869). ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling (PubMed : 27564865).
See full target information UBA7

대체 명칭 보기

UBE1L, UBE2, UBA7, Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog
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Product promise

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