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AB48775

Recombinant Human Ube2L3/UBCH7 protein (Tag Free)

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Recombinant Human Ube2L3/UBCH7 protein (Tag Free) is a Human Full Length protein, in the 1 to 154 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Human Ube2L3/UBCH7 protein (Tag Free) (AB48775)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Ube2L3/UBCH7 protein (Tag Free) (AB48775)

14% SDS-PAGE gel loaded with ab48775
recombinant human Ube2L3/UBCH7 protein
predicted molecular weight 17.9KDa

주요 정보

Purity

>95% SDS-PAGE

Ube2L3/UBCH7 was overexpressed in E.coli and purified by using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P68036-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: 10% Glycerol (glycerin, glycerine), 1.19% HEPES, 0.87% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Proteins & Peptides

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Recombinant human UBE1 protein (Active) (GST tag N-Terminus)

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Proteins & Peptides

AB82791

Recombinant Human STUB1/CHIP protein (Tag Free)

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Proteins & Peptides

AB95927

Recombinant human NEDD4 protein (DDDDK tag N-Terminus)

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Proteins & Peptides

AB269103

Recombinant human UBE2I / UBC9 protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as Ube2L3
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서열 정보

[{"linker":null,"sequence":"MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":154,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P68036","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ube2L3 also known as UBCH7 is an E2 ubiquitin-conjugating enzyme with a molecular weight of approximately 21 kDa. This enzyme participates mechanically in the ubiquitination process where it transfers ubiquitin from an E1 activating enzyme to an E3 ligase mediating protein degradation through the proteasome. Ube2L3 is expressed ubiquitously in various tissues showing higher abundance in the brain heart and skeletal muscle.
Biological function summary

Ube2L3 plays essential roles in protein turnover and cell cycle regulation by functioning within multi-protein complexes. It helps facilitate the attachment of ubiquitin to specific target proteins marking them for degradation. These activities are important for maintaining protein homeostasis and regulation of cell division apoptosis and signal transduction. Ube2L3's role in these biological processes makes it an important component of cellular function and physiology.

Pathways

Ube2L3 is involved in the ubiquitin-proteasome pathway and is also associated with the NF-kB signaling pathway. Its function in these pathways highlights its role in regulating immune responses and inflammatory processes. Interaction with other proteins like E3 ligases including Parkin and Mdm2 shows its involvement in protein quality control and cellular stress responses. Ube2L3's modulation of these pathways is significant for proper cellular responses to environmental cues and stressors.

Ube2L3 associates with conditions like autoimmune diseases and certain cancers. Research links dysfunction in Ube2L3's activity to systemic lupus erythematosus (SLE) where immune regulation is disrupted. Furthermore studies indicate that alterations in its protein interactions particularly with E3 ligases such as Mdm2 might contribute to tumorigenesis by affecting p53 regulation. Understanding these connections helps in elucidating the roles of Ube2L3 in disease mechanisms and may support the development of targeted therapies.
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일반 정보

기능

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine; in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates ubiquitination by the CUL9-RBX1 complex (PubMed : 38605244). In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.

서열 유사성

Belongs to the ubiquitin-conjugating enzyme family.

Post-translational modifications

Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. Autoubiquitinated in vitro (PubMed:22496338).

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제품 프로토콜

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타겟 정보

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine; in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates ubiquitination by the CUL9-RBX1 complex (PubMed : 38605244). In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
See full target information UBE2L3

대체 명칭 보기

UBCE7, UBCH7, UBE2L3, Ubiquitin-conjugating enzyme E2 L3, E2 ubiquitin-conjugating enzyme L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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