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AB177632

Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) (His tag N-Terminus)

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Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 35 to 480 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCRC1

1 이미지
SDS-PAGE - Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) (His tag N-Terminus) (AB177632)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) (His tag N-Terminus) (AB177632)

15% SDS-PAGE analysis of ab177632 (3 μg).

주요 정보

Purity

>85% SDS-PAGE

ab177632 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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Proteins & Peptides

AB172817

Recombinant Human Cytochrome C protein

  • 0
  • 0
Assay Kits

AB109905

MitoTox™ Complex II + III OXPHOS Activity Assay Kit

Functional Studies - MitoTox™ Complex II + III OXPHOS Activity Assay Kit (AB109905)

  • 0
  • 0
Primary Antibodies

AB110252

Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (AB110252)

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  • 1
Proteins & Peptides

AB290069

Recombinant Human PP1A Protein

Mass Spectrometry - Recombinant Human PP1A Protein (AB290069)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSEFTATFAQALQFVPETQVSLLDNGLRVASEQSSQPTCTVGVWIDVGSRFETEKNNGAGYFLEHLAFKGTKNRPGSALEKEVESMGAHLNAYSTREHTAYYIKALSKDLPKAVELLGDIVQNCSLEDSQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF","proteinLength":"Full Length","predictedMolecularWeight":"51.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":480,"aminoAcidStart":35,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P31930","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquinol-Cytochrome C Reductase Core Protein I also known as Core Protein I of the cytochrome c reductase complex functions as part of the mitochondrial electron transport chain. It has a molecular weight of around 52 kDa. The protein plays an integral role within the inner mitochondrial membrane primarily performing mechanical roles in electron transfer. Ubiquinol-Cytochrome C Reductase Core Protein I also goes by its abbreviated symbol UQCRC1 and is expressed broadly across tissues with high energy demands.
Biological function summary

UQCRC1 acts as a core component of Complex III also referred to as Cytochrome bc1 complex. This complex sits centrally within the mitochondrial respiratory chain catalyzing the transfer of electrons from ubiquinol to cytochrome c. By facilitating this electron transfer UQCRC1 aids in generating a proton gradient across the inner mitochondrial membrane essential for ATP synthesis during oxidative phosphorylation.

Pathways

UQCRC1 finds itself prominently embedded in the oxidative phosphorylation and electron transport chain pathways. It directly interacts with other proteins such as cytochrome c and cytochrome reductase emphasizing its cooperative function in maintaining cellular energy balance. The seamless integration of UQCRC1 into these pathways highlights its importance in sustaining efficient mitochondrial operation a critical element for cellular respiration.

UQCRC1 shows connections to conditions involving mitochondrial dysfunction such as Leigh syndrome and mitochondrial myopathy. These disorders sometimes exhibit impaired electron transport impacting ATP production. Proteins like cytochrome c and other cytochrome reductase components often participate in these disorder pathways indicating potential targets for therapeutic interventions that aim to restore or augment mitochondrial function.
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일반 정보

기능

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). Seems to play an important role in the maintenance of proper mitochondrial function in nigral dopaminergic neurons (PubMed : 33141179).

서열 유사성

Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily.

Subcellular localisation

Mitochondrion inner membrane

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제품 프로토콜

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타겟 정보

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). Seems to play an important role in the maintenance of proper mitochondrial function in nigral dopaminergic neurons (PubMed : 33141179).
See full target information UQCRC1
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Product promise

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