AB167714

Recombinant Human uPA protein

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(3 Publications)

Recombinant Human uPA protein is a Human Full Length protein, in the 21 to 431 aa range, expressed in HEK 293 cells, with >92%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU

2 Images

Functional Studies - Recombinant Human uPA protein (AB167714)

FuncS

Supplier Data

Functional Studies - Recombinant Human uPA protein (AB167714)

Immobilized ab167714 at 5 μg/mL (100 μL/well) can bind Biotinylated Human uPAR, His,Avitag with a linear range of 0.3-2 ng/mL

SDS-PAGE - Recombinant Human uPA protein (AB167714)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human uPA protein (AB167714)

SDS-PAGE of reduced ab167714 under reducing (R) condition, stained overnight with Coomassie Blue.

주요 정보

Purity

>92% SDS-PAGE

Less than 1.0 EU per µg by the LAL method.Purified by Immobilized metal affinity chromatography.

Endotoxin 레벨

< 1 EU/µg

발현 시스템

HEK 293 cells

Applications

FuncS, SDS-PAGE

applications

Biologically active

Accession

P00749

Animal free

Carrier free

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

보관 버퍼

pH: 7.4 Constituents: PBS, 0.41% Sodium acetate

storage-buffer

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Please note that the protein is not activated by protease digestion. In vitro activation is generally recommended for higher activity.

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서열 정보

[{"sequence":"SNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL","proteinLength":"Full Length","predictedMolecularWeight":"45 kDa","actualMolecularWeight":null,"aminoAcidEnd":431,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P00749","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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특성 및 보관 정보

제형

Lyophilized

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

False

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일반 정보

기능

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

서열 유사성

Belongs to the peptidase S1 family.

Post-translational modifications

Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.. Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4.

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

See full target information Urokinase-type plasminogen activator

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of physiology. Lung cellular and molecular physiology 322:L593-L606 PubMed35200041

2022

Fibrinogen mediates cadmium-induced macrophage activation and serves as a predictor of cadmium exposure in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fu Jun Li,Ranu Surolia,Pooja Singh,Kevin G Dsouza,Crystal T Stephens,Zheng Wang,Rui-Ming Liu,Sejong Bae,Young-Il Kim,Mohammad Athar,Mark T Dransfield,Veena B Antony

Scientific reports 11:14849 PubMed34290311

2021

CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1.

Applications

Unspecified application

Species

Unspecified reactive species

Sibel Durlanik,Katrin Fundel-Clemens,Coralie Viollet,Heinrich J Huber,Martin Lenter,Kerstin Kitt,Stefan Pflanz

Reproduction (Cambridge, England) 160:853-862 PubMed33112787

2020

The cAMP-ERK1/2 signaling pathway regulates urokinase-type plasminogen activator-induced bovine granulosa cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Yufen Zhao,Boyang Yu,Xinyu Liu,Jitu Hu,Yanyan Yang,Erge Namei,Bingxue Yang,Yue Bai,Yinghong Qian,Haijun Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant Human uPA protein

Functional Studies - Recombinant Human uPA protein (AB167714)

SDS-PAGE - Recombinant Human uPA protein (AB167714)

주요 정보

Purity

Endotoxin 레벨

발현 시스템

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Reconstitution

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

제품 세부 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

일반 정보

기능

서열 유사성

Post-translational modifications

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise