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AB269125

Recombinant Human USP51 protein (GST tag N-Terminus)

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Recombinant Human USP51 protein (GST tag N-Terminus) is a Human Fragment protein, in the 227 to 711 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE.

대체 명칭 보기

Ubiquitin carboxyl-terminal hydrolase 51, Deubiquitinating enzyme 51, Ubiquitin thioesterase 51, Ubiquitin-specific-processing protease 51, USP51

1 이미지
SDS-PAGE - Recombinant Human USP51 protein (Tagged) (AB269125)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human USP51 protein (Tagged) (AB269125)

SDS-PAGE analysis of ab269125.

주요 정보

Purity

>80% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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보완 제품

Primary Antibodies

AB195289

Anti-USP22 antibody [EPR18945]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP22 antibody [EPR18945] (AB195289)

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  • 1
Primary Antibodies

AB167037

Anti-CDCA3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA3 antibody - C-terminal (AB167037)

  • 0
  • 0
Primary Antibodies

AB126604

Anti-USP28 antibody [EPR4249(2)]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-USP28 antibody [EPR4249(2)] (AB126604)

  • 0
  • 0
Primary Antibodies

AB140601

Anti-Ubiquitin (linkage-specific K48) antibody [EP8589]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Ubiquitin (linkage-specific K48) antibody [EP8589] (AB140601)

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  • 10
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"80 kDa","actualMolecularWeight":"82 kDa","aminoAcidEnd":711,"aminoAcidStart":227,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q70EK9","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

USP51 also known as Ubiquitin Specific Peptidase 51 functions as a deubiquitinating enzyme. It has a mass of approximately 96 kDa. This protein regulates the ubiquitin-proteasome pathway by removing ubiquitin molecules from ubiquitinated substrates. USP51 gets predominantly expressed in tissues such as the testis and the brain. Through its enzymatic activity USP51 maintains protein homeostasis which is essential for various cellular processes.
Biological function summary

Ubiquitin Specific Peptidase 51 plays a role in DNA damage repair and cell cycle regulation. It forms part of multiprotein complexes where it collaborates with components like BRCA1 and RAD51 to maintain genomic stability. This activity ensures the cell's integrity and function. Moreover USP51 interacts with other deubiquitinating enzymes indicating a coordinated action in cellular maintenance.

Pathways

The activity of USP51 integrates into the DNA damage response and cell cycle checkpoints. It contributes to the homologous recombination repair pathway alongside proteins like RAD51 ensuring proper repair of DNA double-strand breaks. Additionally USP51 influences the Wnt signaling pathway where it affects the stability of proteins like β-catenin which are integral to cell proliferation and differentiation.

The regulation by USP51 impacts cancer development and neurodegenerative disorders. Abnormal expression or mutation of USP51 has links to breast cancer due to its influence on the BRCA1-associated DNA repair pathway. In neurodegenerative diseases like Alzheimer's USP51 may alter protein aggregation through its effect on ubiquitin pathways potentially intersecting with proteins such as tau. These connections present USP51 as a significant target for therapeutic research and development.
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일반 정보

기능

Specifically deubiquitinates 'Lys-14' (H2AK13Ub) and 'Lys-16'(H2AK15Ub) of histone H2A regulating the DNA damage response at double-strand breaks (DSBs) (PubMed : 27083998, PubMed : 33022275). USP51 is recruited to chromatin after DNA damage and regulates the dynamic assembly/disassembly of TP53BP1 and BRCA1. Functions in DNA double-strand break repair also by mediating the deubiquitination and subsequent stabilization of DGCR8, leading to the recruitment of DGCR8 binding partners to double strand breaks such as RNF168 or MDC1 (PubMed : 34188037). In addition, promotes the deubiquitination and stabilization of the transcriptional repressor ZEB1 (PubMed : 29119051).

서열 유사성

Belongs to the peptidase C19 family.

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제품 프로토콜

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타겟 정보

Specifically deubiquitinates 'Lys-14' (H2AK13Ub) and 'Lys-16'(H2AK15Ub) of histone H2A regulating the DNA damage response at double-strand breaks (DSBs) (PubMed : 27083998, PubMed : 33022275). USP51 is recruited to chromatin after DNA damage and regulates the dynamic assembly/disassembly of TP53BP1 and BRCA1. Functions in DNA double-strand break repair also by mediating the deubiquitination and subsequent stabilization of DGCR8, leading to the recruitment of DGCR8 binding partners to double strand breaks such as RNF168 or MDC1 (PubMed : 34188037). In addition, promotes the deubiquitination and stabilization of the transcriptional repressor ZEB1 (PubMed : 29119051).
See full target information USP51
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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