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AB269138

Recombinant human Wwp2 protein (Active) (GST tag N-Terminus)

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Recombinant human Wwp2 protein (Active) (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 870 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human Wwp2 protein (Active) (GST tag N-Terminus) (AB269138)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Wwp2 protein (Active) (GST tag N-Terminus) (AB269138)

The specific activity of ab269138 was 53 nmol/min/mg in an assay using wild-type ubiquitin as substrate.

SDS-PAGE - Recombinant human Wwp2 protein (Active) (GST tag N-Terminus) (AB269138)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Wwp2 protein (Active) (GST tag N-Terminus) (AB269138)

SDS-PAGE analysis of ab269138.

주요 정보

Purity

>90% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab269138 was 53 nmol/min/mg in an assay using wild-type ubiquitin as substrate.

Accession

O00308-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
  • 0
Proteins & Peptides

AB163053

Recombinant Human Fbxw7 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Fbxw7 protein (GST tag N-Terminus) (AB163053)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB68071

Anti-TRIM22 antibody

  • 0
  • 0
Primary Antibodies

AB202445

Anti-Smad2 + Smad3 antibody [EPR19557-4] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Smad2 + Smad3 antibody [EPR19557-4] - ChIP Grade (AB202445)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIILNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENMQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGNVPNGSALTDGSQLPSRDSSGTAVAPENRHQPPSTNCFGGRSRTHRHSGASARTTPATGEQSPGARSRHRQPVKNSGHSGLANGTVNDEPTTATDPEEPSVVGVTSPPAAPLSVTPNPNTTSLPAPATPAEGEEPSTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":870,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O00308","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wwp2 also known as WWP2 or ubiquitin protein ligase E3 component N-terminal homolog 2 is a member of the NEDD4-like E3 ubiquitin ligases. It has a molecular weight of approximately 104 kDa. This protein facilitates the attachment of ubiquitin molecules to substrate proteins tagging them for proteasomal degradation or for regulation of their activity localization or interactions. Wwp2 is expressed in various tissues with higher expression in lung heart muscle and kidney indicating a broad functional range.
Biological function summary

Wwp2 plays a critical role in cellular processes like growth division and differentiation by regulating the stability of key signaling proteins. It functions as part of protein complexes that include WW domain-containing proteins which enables it to interact with multiple substrates. Through its ubiquitination activity Wwp2 influences pathways involved in the maintenance of cellular homeostasis and response to stimuli.

Pathways

Wwp2 has significant involvement in the TGF-beta signaling pathway and the Wnt signaling pathway. In the TGF-beta pathway Wwp2 mediates the degradation of SMAD2 and SMAD7 which are central to the regulation of this signaling cascade. Wwp2 also interacts with proteins like SMURF2 and RNF11 within these pathways promoting fine-tuned control of signal transduction ultimately influencing development and differentiation processes.

Wwp2 is linked to conditions such as cancer and cardiac hypertrophy. Dysregulation of Wwp2 can lead to aberrations in the TGF-beta pathway contributing to oncogenic processes and proliferation of tumor cells. Additionally Wwp2's role in the Wnt pathway associates it with cardiac hypertrophy where altered signaling may disturb cardiac muscle cell growth and function. Noteworthy Wwp2's interaction with proteins like SMAD7 in these contexts offers further insight into its pathological relevance.
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일반 정보

기능

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.

Post-translational modifications

Autoubiquitinated. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome.

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제품 프로토콜

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타겟 정보

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.
See full target information WWP2

대체 명칭 보기

NEDD4-like E3 ubiquitin-protein ligase WWP2, Atrophin-1-interacting protein 2, HECT-type E3 ubiquitin transferase WWP2, WW domain-containing protein 2, AIP2, WWP2
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Product promise

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