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AB164876

Recombinant Human YTHDC1 protein (GST tag N-Terminus)

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Recombinant Human YTHDC1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 709 aa range, expressed in Wheat germ, suitable for ELISA, WB.

대체 명칭 보기

KIAA1966, YT521, YTHDC1, YTH domain-containing protein 1, Splicing factor YT521, YT521-B

1 이미지
SDS-PAGE - Recombinant Human YTHDC1 protein (GST tag N-Terminus) (AB164876)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human YTHDC1 protein (GST tag N-Terminus) (AB164876)

ab164876 on a 12.5% SDS-PAGE stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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보완 제품

Primary Antibodies

AB259990

Anti-YTHDC1 antibody [EPR21821-213]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-YTHDC1 antibody [EPR21821-213] (AB259990)

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Proteins & Peptides

AB132957

Recombinant Human Prostaglandin E Receptor EP2/PTGER2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Prostaglandin E Receptor EP2/PTGER2 protein (GST tag N-Terminus) (AB132957)

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  • 0
Proteins & Peptides

AB166176

Recombinant Human WDR62 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human WDR62 protein (GST tag N-Terminus) (AB166176)

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  • 0
Assay Kits

AB212166

Human BDNF ELISA Kit

SimpleStep

ELISA - Human BDNF ELISA Kit (AB212166)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MAADSREEKDGELNVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRKSDRMESTDTKRQKPSVHSRQLVSKPLSSSVSNNKRIVSTKGKSATEYKNEEYQRSERNKRLDADRKIRLSSSASREPYKNQPEKTCVRKRDPERRAKSPTPDGSERIGLEVDRRASRSSQSSKEEVNSEEYGSDHETGSSGSSDEQGNNTENEEEGVEEDVEEDEEVEEDAEEDEEVDEDGEEEEEEEEEEEEEEEEEEEEYEQDERDQKEEGNDYDTRSEASDSGSESVSFTDGSVRSGSGTDGSDEKKKERKRARGISPIVFDRSGSSASESYADQTSKLKYVLQDARFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQVIHKMRHKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDRRFSGVRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGMEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERLCDRDRDRGERGRYRR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":709,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96MU7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

YTHDC1 also known as YT521-B is a nuclear protein involved in mRNA processing. It belongs to the YTH domain family recognized for binding m6A-modified RNA. The protein has a mass of approximately 82 kDa. Expression of YTHDC1 occurs mainly in the nucleus but may also be found in other cellular compartments. Its widespread presence includes tissues such as the brain and liver suggesting its diverse functional roles.
Biological function summary

YTHDC1 operates in the regulation of mRNA splicing and transportation. It associates with spliceosome components to influence alternative splicing decisions. YTHDC1 part of the m6A reader complex recognizes the m6A methylation mark on RNA aiding in the control of mRNA fate. Additionally its interactions with certain proteins affect RNA stability and translation essential for proper gene expression regulation.

Pathways

YTHDC1 plays a significant role in RNA metabolism and the cellular response to stress. Involvement in the mRNA splicing pathway highlights its contribution to gene expression regulation. YTHDC1 interacts with proteins like SRSF3 and SRFS10 which modulate RNA splicing and stability. Another pathway includes its role in the cellular stress response where YTHDC1 helps maintain RNA integrity linking it to adaptive responses in cells.

YTHDC1 captures interest regarding cancer and neurodegenerative diseases. Abnormal expression levels or mutations in YTHDC1 have associations with various cancer types hinting at its role in tumorigenesis. Alterations in YTHDC1 expression impact splicing patterns and gene expression potentially influencing cancer progression. Additionally YTHDC1's connection to neurodegeneration involves proteins such as FUS and TDP-43 which are associated with disorders like amyotrophic lateral sclerosis (ALS) suggesting that YTHDC1 may play a role in RNA metabolism dysregulation observed in these diseases.
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일반 정보

기능

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed : 25242552, PubMed : 26318451, PubMed : 26876937, PubMed : 28984244). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed : 25242552, PubMed : 26318451). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed : 26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed : 26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping : this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed : 26876937). May also regulate alternative splice site selection (PubMed : 20167602). Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3 : interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity). Also recognizes and binds m6A on other RNA molecules (PubMed : 27602518). Involved in random X inactivation mediated by Xist RNA : recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed : 27602518). Also recognizes and binds m6A-containing single-stranded DNA (PubMed : 32663306). Involved in germline development : required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing (By similarity). Independently of its N6-methyladenosine-containing RNA reader activity, it regulates PPARG stability by preventing its interaction with the E3 ubiquitin ligase ARIH2, thereby protecting it from ubiquitin-mediated proteasomal degradation and contributing to brown adipose tissue (BAT) development and energy homeostasis (By similarity).

Post-translational modifications

Tyrosine phosphorylated.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed : 25242552, PubMed : 26318451, PubMed : 26876937, PubMed : 28984244). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed : 25242552, PubMed : 26318451). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed : 26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed : 26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping : this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed : 26876937). May also regulate alternative splice site selection (PubMed : 20167602). Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3 : interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity). Also recognizes and binds m6A on other RNA molecules (PubMed : 27602518). Involved in random X inactivation mediated by Xist RNA : recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed : 27602518). Also recognizes and binds m6A-containing single-stranded DNA (PubMed : 32663306). Involved in germline development : required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing (By similarity). Independently of its N6-methyladenosine-containing RNA reader activity, it regulates PPARG stability by preventing its interaction with the E3 ubiquitin ligase ARIH2, thereby protecting it from ubiquitin-mediated proteasomal degradation and contributing to brown adipose tissue (BAT) development and energy homeostasis (By similarity).
See full target information YTHDC1
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Product promise

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