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AB276746

Recombinant Mouse ARSA/ASA protein (His tag)

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Recombinant Mouse ARSA/ASA protein (His tag) is a Mouse Full Length protein, in the 1 to 506 aa range, expressed in HEK 293 cells, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

대체 명칭 보기

As2, Arsa, Arylsulfatase A, ASA, Cerebroside-sulfatase

1 이미지
SDS-PAGE - Recombinant Mouse ARSA/ASA protein (His tag) (AB276746)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse ARSA/ASA protein (His tag) (AB276746)

SDS-PAGE analysis of ab276746

주요 정보

Purity

>97% SDS-PAGE

Endotoxin 레벨

< 1 EU/µg

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Mouse

보관 버퍼

pH: 7.4 Constituents: 0.3% Tris, 0.00087% Sodium chloride

storage-buffer

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보완 제품

Primary Antibodies

AB174844

Anti-ARSA/ASA antibody [EPR11039]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSA/ASA antibody [EPR11039] (AB174844)

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Primary Antibodies

AB32138

Anti-PARP1 antibody [E102]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-PARP1 antibody [E102] (AB32138)

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Proteins & Peptides

AB116968

Recombinant Human APOF protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human APOF protein (GST tag N-Terminus) (AB116968)

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  • 0
Proteins & Peptides

AB186079

Recombinant Human TRIB1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human TRIB1 protein (AB186079)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MALGTLFLALAAGLSTASPPNILLIFADDLGYGDLGSYGHPSSTTPNLDQLAEGGLRFTDFYVPVSLCTPSRAALLTGRLPVRSGMYPGVLGPSSQGGLPLEEVTLAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPDIPCKGGCDQGLVPIPLLANLTVEAQPPWLPGLEARYVSFSRDLMADAQRQGRPFFLYYASHHTHYPQFSGQSFTKRSGRGPFGDSLMELDGAVGALMTTVGDLGLLEETLVIFTADNGPELMRMSNGGCSGLLRCGKGTTFEGGVREPALVYWPGHITPGVTHELASSLDLLPTLAALTGAPLPNVTLDGVDISPLLLGTGKSPRKSVFFYPPYPDEIHGVFAVRNGKYKAHFFTQGSAHSDTTSDPACHAANRLTAHEPPLLYDLSQDPGENYNVLESIEGVSPEALQALKHIQLLKAQYDAAMTFGPSQIAKGEDPALQICCQPSCTPHPVCCHCPGSQS","proteinLength":"Full Length","predictedMolecularWeight":"53.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":506,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P50428","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ARSA protein also known as arylsulfatase A or ASA protein functions mechanically as a lysosomal enzyme responsible for the cleavage of cerebroside sulfate into cerebroside and sulfate. It belongs to a family of enzymes important for the hydrolysis of sulfate esters and has a molecular mass of about 62 kDa. This enzyme is expressed mainly in the liver and kidney but also in the brain where it plays important roles in normal functioning.
Biological function summary

ARSA is essential in the breakdown of sulfatides which are components of myelin sheaths in the central nervous system. It functions as part of a lysosomal complex involving cooperators like saposin B which facilitates its interaction with sulfatide substrates. By breaking down sulfatides ARSA helps in cellular membrane maintenance and recycling contributing to neurological function and stability.

Pathways

ARSA is mainly involved in the sphingolipid metabolism pathway. This pathway manages the degradation of sphingolipids important for maintaining neuron and cell membrane integrity. ARSA also interacts with proteins such as galactosylceramidase as both play roles in lipid recycling and are essential for maintaining cell health within the nervous system.

ARSA's deficiency is a well-known factor in metachromatic leukodystrophy (MLD) a genetic disorder leading to progressive leukodystrophy. This condition results from sulfatide accumulation in the nervous system caused by insufficient ARSA activity. The disease involves other proteins such as saposin B which when dysfunctional exacerbate ARSA-related pathologies and symptoms. Another disorder related to defective ARSA function includes chronic kidney disease where compromised sulfatide metabolism further effects cellular processes.
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일반 정보

기능

Hydrolyzes cerebroside sulfate.

서열 유사성

Belongs to the sulfatase family.

Post-translational modifications

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD).

Subcellular localisation

Lysosome

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제품 프로토콜

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타겟 정보

Hydrolyzes cerebroside sulfate.
See full target information Arsa
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Product promise

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