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AB108574

Recombinant Mouse DLK-1 protein (Fc fragment) (Human IgG1 Fc tag C-Terminus)

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Recombinant Mouse DLK-1 protein (Fc fragment) (Human IgG1 Fc tag C-Terminus) is a Mouse Fragment protein, in the 1 to 305 aa range, expressed in HEK 293 cells, with >90%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant Mouse DLK-1 protein (Fc fragment) (AB108574)
  • FuncS

Supplier Data

Functional Studies - Recombinant Mouse DLK-1 protein (Fc fragment) (AB108574)

Induction of SOX-9 with the treatment of recombinant mouse DLK-1-Fc (ab108574).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of ab108574 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-rabbit Sox-9 and anti-rabbit GAPDH.

1=0 min; 2=10 min; 3=30 min; 4=1 h; 5=2 h; 6=3 h; 7=4 h; 8=24 h.

주요 정보

Purity

>90% SDS-PAGE

ab108574 is 0.2µm filtered

Endotoxin 레벨

< 0.1 EU/µg

발현 시스템

HEK 293 cells

Tags

Human IgG1 Fc tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

No

Accession

Q09163-1

Animal free

No

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in 100 µL of water

보관 버퍼

Constituents: PBS

storage-buffer

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보완 제품

Primary Antibodies

AB210471

Anti-DLK-1 antibody [EPR19830]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (AB210471)

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  • 4
Primary Antibodies

AB306534

Alexa Fluor® 647 Anti-c-Kit antibody [EPR24708-25]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-c-Kit antibody [EPR24708-25] (AB306534)

  • 0
  • 0
Proteins & Peptides

AB191954

Recombinant Mouse Ephrin A4 protein

  • 0
  • 0
Proteins & Peptides

AB181923

Recombinant Human LANCL1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human LANCL1 protein (His tag N-Terminus) (AB181923)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"5 µg/mL", "notes":"<p></p>" } } }
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제품 세부 정보

Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"75 kDa","actualMolecularWeight":null,"aminoAcidEnd":305,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q09163","tags":[{"tag":"Human IgG1 Fc","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
Biological function summary

DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.

Pathways

Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.

DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.
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일반 정보

기능

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.

Post-translational modifications

N- and O-glycosylated.

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제품 프로토콜

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타겟 정보

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
See full target information Dlk1

대체 명칭 보기

Dlk, Pref1, Scp-1, Dlk1, Protein delta homolog 1, DLK-1, Adipocyte differentiation inhibitor protein, Preadipocyte factor 1, Pref-1
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Product promise

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