JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB281800

Recombinant mouse IL-10 protein (Active)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Recombinant mouse IL-10 protein (Active) is a Mouse Full Length protein, in the 19 to 178 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

대체 명칭 보기

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

4 이미지
Functional Studies - Recombinant mouse IL-10 protein (Active) (AB281800)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse IL-10 protein (Active) (AB281800)

Functional analysis of ab281800.

Fully biologically active determined by dose dependent increase in proliferation of MC/9 cells.

ED50 for this effect is ≤ 0.330 ng/ml corresponding to a specific activity of 3.03 X 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3387982-1

Mass Spectrometry - Recombinant mouse IL-10 protein (Active) (AB281800)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse IL-10 protein (Active) (AB281800)

Mass determination by ESI-TOF.

Predicted MW is 18810.75 Da. (+/- 10 Da by ESI-TOF). Observed MW is 18812.07 Da.

HPLC - Recombinant mouse IL-10 protein (Active) (AB281800)
  • HPLC

Supplier Data

HPLC - Recombinant mouse IL-10 protein (Active) (AB281800)

HPLC analysis of ab281800.

SDS-PAGE - Recombinant mouse IL-10 protein (Active) (AB281800)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse IL-10 protein (Active) (AB281800)

SDS-PAGE analysis of ab281800.

주요 정보

Purity

>95% SDS-PAGE

Equal or greater than 95% by HPLC

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

Cell Culture, FuncS, SDS-PAGE, HPLC, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by dose dependent increase in proliferation of MC/9 cells.

ED50 for this effect is ≤ 0.330 ng/ml corresponding to a specific activity of 3.03 X 106 units/mg.

Accession

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

보완 제품

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Proteins & Peptides

AB259406

Recombinant mouse IL-4 protein (Active)

Premium Bioactive

Functional Studies - Recombinant mouse IL-4 protein (Active) (AB259406)

  • 0
  • 0
Proteins & Peptides

AB259410

Recombinant human TNF alpha protein (Active)

Premium Bioactive

Flow Cytometry - Recombinant human TNF alpha protein (Active) (AB259410)

  • 0
  • 0
Proteins & Peptides

AB259377

Recombinant Human Interferon gamma protein (Active)

Premium Bioactive

Functional Studies - Recombinant Human Interferon gamma protein (Active) (AB259377)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

서열 정보

[{"linker":null,"sequence":"SRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS","proteinLength":"Full Length","predictedMolecularWeight":"18.81 kDa","actualMolecularWeight":"18.81 kDa","aminoAcidEnd":178,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P18893","tags":[]}]
style
left-column

특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
style
left-column
style
left-column

일반 정보

기능

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).

서열 유사성

Belongs to the IL-10 family.

style
left-column

제품 프로토콜

style
left-column

타겟 정보

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).
See full target information Il10
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com