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AB281809

Recombinant mouse IL-22 protein (Active)

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Recombinant mouse IL-22 protein (Active) is a Mouse Full Length protein, in the 25 to 179 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

대체 명칭 보기

Il22a, Iltif, Iltifa, Il22, Interleukin-22, IL-22, IL-10-related T-cell-derived-inducible factor, IL-TIF alpha, Interleukin-22a, IL-TIF, IL-22a

4 이미지
Mass Spectrometry - Recombinant mouse IL-22 protein (Active) (AB281809)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse IL-22 protein (Active) (AB281809)

Mass determination by ESI-TOF.

Predicted MW is 16697.32 Da. (+/- 10 Da by ESI-TOF). Observes MW is 16700.44 Da.

Functional Studies - Recombinant mouse IL-22 protein (Active) (AB281809)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse IL-22 protein (Active) (AB281809)

Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.

ED50 is ≤530 pg/ml, corresponding to a specific activity of 1.88 x 106 units /mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3384879-2

SDS-PAGE - Recombinant mouse IL-22 protein (Active) (AB281809)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse IL-22 protein (Active) (AB281809)

SDS-PAGE analysis of ab281809.

HPLC - Recombinant mouse IL-22 protein (Active) (AB281809)
  • HPLC

Supplier Data

HPLC - Recombinant mouse IL-22 protein (Active) (AB281809)

HPLC analysis of ab281809.

주요 정보

Purity

>95% SDS-PAGE

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

FuncS, HPLC, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.

ED50 is ≤530 pg/ml, corresponding to a specific activity of 1.88 x 106 units /mg.

Accession

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

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Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"LPVNTRCKLEVSNFQQPYIVNRTFMLAKEASLADNNTDVRLIGEKLFRGVSAKDQCYLMKQVLNFTLEDVLLPQSDRFQPYMQEVVPFLTKLSNQLSSCHISGDDQNIQKNVRRLKETVKKLGESGEIKAIGELDLLFMSLRNACV","proteinLength":"Full Length","predictedMolecularWeight":"16.69 kDa","actualMolecularWeight":"16.7 kDa","aminoAcidEnd":179,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9JJY9","tags":[]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
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일반 정보

기능

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed : 33852830, PubMed : 35525330). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed : 33912578). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (PubMed : 24742671). Promotes epithelial cell spreading (PubMed : 24742671).

서열 유사성

Belongs to the IL-10 family.

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제품 프로토콜

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타겟 정보

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed : 33852830, PubMed : 35525330). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed : 33912578). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (PubMed : 24742671). Promotes epithelial cell spreading (PubMed : 24742671).
See full target information Il22
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Viruses 17: PubMed40006939

2025

ILC3 Function as a Double-Edged Sword in EV71 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Zhang,Linlin Bao,Feifei Qi,Qi Lv,Fengdi Li,Chuan Qin
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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